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Microdialysis sampling method for evaluation of binding kinetics of small molecules to macromolecules
Wang, Hailin1; Wang, Zhixin1; Liu, Meiling1; Zou, Hanfa2; Zou HF(邹汉法); Zou HF(邹汉法)
刊名ANALYTICAL CHEMISTRY
2008-04-15
ISSN0003-2700
DOI10.1021/ac702066x
80期:8页:2993-2999
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名2;4
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]SURFACE-PLASMON RESONANCE ; HUMAN SERUM-ALBUMIN ; NONEQUILIBRIUM CAPILLARY-ELECTROPHORESIS ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; EQUILIBRIUM MIXTURES ; INTRACEREBRAL MICRODIALYSIS ; NONCOMPETITIVE INHIBITORS ; QUANTITATIVE-ANALYSIS ; PROTEIN INTERACTIONS ; STATIONARY-PHASE
英文摘要Here we present an application of microdialysis sampling for evaluation of the binding kinetics of small molecules to macromolecules. It is label-free, and no immobilization of any interaction partner is required. The method was established by the coupling of a binding reaction with a membrane transport in a miniature and dynamic microdialysis sampling system. A theoretical model was established to describe the quantitative relationship between the binding kinetics of small ligands to macromolecules and the enhanced mass transport of small ligands and was applied to estimate the binding kinetics. To demonstrate the proof-of-principle, we examined the binding kinetics of an abundant plasma protein human serum albumin (HSA) and a representative drug ketoprofen as an example. The primary binding constant of ketoprofen to HSA was estimated as 1.63 ( +/- 0.12) x 106 M-1. The estimated association and dissociation rate constants (k(1) and k(-1)) were about 3.71 x 10(5) M-1 s(-1) and 0.227 s(-1), respectively. The results suggest a fast binding of ketoprofen to HSA and a fast dissociation of the formed complex, which are consistent with the reversible binding property of drug and HSA (k(-1) in the order of s(-1)). This is the first report on binding-kinetics measurement using microdialysis sampling.
语种英语
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WOS记录号WOS:000254969100045
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/100005
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromat Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Wang, Hailin,Wang, Zhixin,Liu, Meiling,et al. Microdialysis sampling method for evaluation of binding kinetics of small molecules to macromolecules[J]. ANALYTICAL CHEMISTRY,2008,80(8):2993-2999.
APA Wang, Hailin,Wang, Zhixin,Liu, Meiling,Zou, Hanfa,邹汉法,&邹汉法.(2008).Microdialysis sampling method for evaluation of binding kinetics of small molecules to macromolecules.ANALYTICAL CHEMISTRY,80(8),2993-2999.
MLA Wang, Hailin,et al."Microdialysis sampling method for evaluation of binding kinetics of small molecules to macromolecules".ANALYTICAL CHEMISTRY 80.8(2008):2993-2999.
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