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题名: Purification and characterization of a novel glycosidase from the China white jade snail (Achatina fulica) showing transglycosylation activity
作者: Hu, Ying1, 2;  Luan, Hongwei1, 2;  Zhou, Kun1, 2;  Ge, Guangbo1, 2;  Yang, Shengli1;  Yang, Ling1
通讯作者: 杨凌
关键词: beta-D-glycosidase ;  transglycosylation ;  thermal and pH stability ;  broad specificity ;  alkyl glycosides ;  enzyme purification
刊名: ENZYME AND MICROBIAL TECHNOLOGY
发表日期: 2008-07-07
DOI: 10.1016/j.enzmictec.2008.02.010
卷: 43, 期:1, 页:35-42
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1806
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: A broad specificity beta-D-glycosidase, designated G1, was purified to homogeneity from the viscera of the China white jade snail (Achatina fulica). The enzyme was a monomeric protein with molecular weight of 115 kDa. G I has broad glycone specificity towards p-nitrophenyl derivatives Of beta-D-glucose, beta-D-fucose, beta-D-galactose, alpha-D-glucose and some disaccharides. The optimum pH and temperature are 5.5 and 55 degrees C, respectively. It was stable over a wide pH range (5-10 at 30 degrees C for 24h) and against a relatively high temperature (50 degrees C for 4h). Moreover, it was also stable and active in the presence of various alcohols. With pNPGlu, pNPFuc and cellobiose as donor, G I showed high transglycosylation activity. Six transglycosylation products were isolated from the reaction mixture containing 20% alcohol as glycoside acceptor using a preparative thin layer chromatography (preparative TLC) and identified as alkyl-glucos ides and alkyl-fucosides by mass spectrometry (MS) analysis. Combining the high alcohol tolerance, moderate temperature and pH stability and alkyl glycosides production efficiency through transglycosylation, G I can be considered to be a promising candidate for the production of various alkyl glycosides. (C) 2008 Elsevier Inc. All rights reserved.
关键词[WOS]: BETA-D-GLUCOSIDASE ;  CATALYZED CONDENSATION ;  THERMOPHILIC FUNGUS ;  ESCHERICHIA-COLI ;  ALKYL GLUCOSIDES ;  BIPHASIC SYSTEM ;  GALACTOSIDASE ;  FUCOSIDASE ;  HYDROLASE ;  DONOR
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000257154400006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100019
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
Hu, Ying,Luan, Hongwei,Zhou, Kun,et al. Purification and characterization of a novel glycosidase from the China white jade snail (Achatina fulica) showing transglycosylation activity[J]. ENZYME AND MICROBIAL TECHNOLOGY,2008,43(1):35-42.
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