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题名: Optimization of seeding density in microencapsulated recombinant CHO cell culture
作者: Zhang, Y.1, 2;  Zhou, J.1, 2;  Zhang, X.1, 2;  Yu, W.1;  Guo, X.1;  Wang, W.1;  Ma, X.1
通讯作者: 马小军
关键词: microencapsulation ;  seeding density ;  recombinant CHO cell ;  endostatin
刊名: CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY
发表日期: 2008-03-01
卷: 22, 期:1, 页:105-111
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Engineering
英文摘要: Microencapsulation technology is an alternative large-scale mammalian cell culture method. The semi-permeable membrane of the microcapsule allows free diffusion of nutrients, oxygen and toxic metabolites to support cell growth, and the microcapsule membrane can protect the cells from the mechanical damage of shear forces associated with agitation and aeration. Many polymers have been used to make microcapsules, such as chitosan, polyacrylates, alginate, polyamino, acids, and polyamides. One of these microcapsules is Alginate-polylysine-alginate (APA) microcapsule. Using the electrostatic droplet generator and polyelectrolyte complexation, we developed APA microcapsules with a diameter of 300 mu m and assessed the effect of the seeding density on microencapsulated recombinant Chinese hamster ovary (CHO) cell growth, metabolism and endostatin production. The results showed that the cell growth was the best and endostatin production was the highest when the seeding density was 3 . 10(6) cells mL(-1) microcapsule, at which the maximal cell density reached 1.82 . 10(7) cells mL(-1) microcapsule and endostatin production reached 12.3 mu g mL(-1) at day 9. The energy metabolism was more efficient when the seeding density was 3 . 106 cells mL(-1) microcapsule, at which more glucose and glutamine were utilized for biosynthesis and less lactate and ammonium were produced.
关键词[WOS]: ANTIBODY-PRODUCTION ;  MAMMALIAN-CELLS ;  HYBRIDOMA CELLS ;  METABOLISM ;  GROWTH
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000255082500011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100037
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhang, Y.,Zhou, J.,Zhang, X.,et al. Optimization of seeding density in microencapsulated recombinant CHO cell culture[J]. CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY,2008,22(1):105-111.
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