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Optimization of seeding density in microencapsulated recombinant CHO cell culture
Zhang, Y.1,2; Zhou, J.1,2; Zhang, X.1,2; Yu, W.1; Guo, X.1; Wang, W.1; Ma, X.1; Ma XJ(马小军); Ma XJ(马小军)
关键词Microencapsulation Seeding Density Recombinant Cho Cell Endostatin
刊名CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY
2008-03-01
22期:1页:105-111
收录类别SCI
文章类型Article
部门归属18
项目归属1802
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]ANTIBODY-PRODUCTION ; MAMMALIAN-CELLS ; HYBRIDOMA CELLS ; METABOLISM ; GROWTH
英文摘要Microencapsulation technology is an alternative large-scale mammalian cell culture method. The semi-permeable membrane of the microcapsule allows free diffusion of nutrients, oxygen and toxic metabolites to support cell growth, and the microcapsule membrane can protect the cells from the mechanical damage of shear forces associated with agitation and aeration. Many polymers have been used to make microcapsules, such as chitosan, polyacrylates, alginate, polyamino, acids, and polyamides. One of these microcapsules is Alginate-polylysine-alginate (APA) microcapsule. Using the electrostatic droplet generator and polyelectrolyte complexation, we developed APA microcapsules with a diameter of 300 mu m and assessed the effect of the seeding density on microencapsulated recombinant Chinese hamster ovary (CHO) cell growth, metabolism and endostatin production. The results showed that the cell growth was the best and endostatin production was the highest when the seeding density was 3 . 10(6) cells mL(-1) microcapsule, at which the maximal cell density reached 1.82 . 10(7) cells mL(-1) microcapsule and endostatin production reached 12.3 mu g mL(-1) at day 9. The energy metabolism was more efficient when the seeding density was 3 . 106 cells mL(-1) microcapsule, at which more glucose and glutamine were utilized for biosynthesis and less lactate and ammonium were produced.
语种英语
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WOS记录号WOS:000255082500011
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/100037
专题中国科学院大连化学物理研究所
通讯作者Ma XJ(马小军); Ma XJ(马小军)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Zhang, Y.,Zhou, J.,Zhang, X.,et al. Optimization of seeding density in microencapsulated recombinant CHO cell culture[J]. CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY,2008,22(1):105-111.
APA Zhang, Y..,Zhou, J..,Zhang, X..,Yu, W..,Guo, X..,...&马小军.(2008).Optimization of seeding density in microencapsulated recombinant CHO cell culture.CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY,22(1),105-111.
MLA Zhang, Y.,et al."Optimization of seeding density in microencapsulated recombinant CHO cell culture".CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 22.1(2008):105-111.
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