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Optimization of seeding density in microencapsulated recombinant CHO cell culture
Zhang, Y.1,2; Zhou, J.1,2; Zhang, X.1,2; Yu, W.1; Guo, X.1; Wang, W.1; Ma, X.1; Ma XJ(马小军); Ma XJ(马小军)
KeywordMicroencapsulation Seeding Density Recombinant Cho Cell Endostatin
Source PublicationCHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY
2008-03-01
Volume22Issue:1Pages:105-111
Indexed BySCI
SubtypeArticle
Department18
Funding Project1802
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS KeywordANTIBODY-PRODUCTION ; MAMMALIAN-CELLS ; HYBRIDOMA CELLS ; METABOLISM ; GROWTH
AbstractMicroencapsulation technology is an alternative large-scale mammalian cell culture method. The semi-permeable membrane of the microcapsule allows free diffusion of nutrients, oxygen and toxic metabolites to support cell growth, and the microcapsule membrane can protect the cells from the mechanical damage of shear forces associated with agitation and aeration. Many polymers have been used to make microcapsules, such as chitosan, polyacrylates, alginate, polyamino, acids, and polyamides. One of these microcapsules is Alginate-polylysine-alginate (APA) microcapsule. Using the electrostatic droplet generator and polyelectrolyte complexation, we developed APA microcapsules with a diameter of 300 mu m and assessed the effect of the seeding density on microencapsulated recombinant Chinese hamster ovary (CHO) cell growth, metabolism and endostatin production. The results showed that the cell growth was the best and endostatin production was the highest when the seeding density was 3 . 10(6) cells mL(-1) microcapsule, at which the maximal cell density reached 1.82 . 10(7) cells mL(-1) microcapsule and endostatin production reached 12.3 mu g mL(-1) at day 9. The energy metabolism was more efficient when the seeding density was 3 . 106 cells mL(-1) microcapsule, at which more glucose and glutamine were utilized for biosynthesis and less lactate and ammonium were produced.
Language英语
URL查看原文
WOS IDWOS:000255082500011
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Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/100037
Collection中国科学院大连化学物理研究所
Corresponding AuthorMa XJ(马小军); Ma XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Y.,Zhou, J.,Zhang, X.,et al. Optimization of seeding density in microencapsulated recombinant CHO cell culture[J]. CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY,2008,22(1):105-111.
APA Zhang, Y..,Zhou, J..,Zhang, X..,Yu, W..,Guo, X..,...&马小军.(2008).Optimization of seeding density in microencapsulated recombinant CHO cell culture.CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY,22(1),105-111.
MLA Zhang, Y.,et al."Optimization of seeding density in microencapsulated recombinant CHO cell culture".CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 22.1(2008):105-111.
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