中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity
作者: Ma, Junfeng1, 2;  Liang, Zhen1;  Qiao, Xiaoqiang1, 2;  Deng, Qiliang1;  Tao, Dingyin1, 2;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
刊名: ANALYTICAL CHEMISTRY
发表日期: 2008-04-15
DOI: 10.1021/ac702343a
卷: 80, 期:8, 页:2949-2956
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A novel kind of immobilized trypsin reactor based on organic-inorganic hybrid silica monoliths has been developed. With the presence of cetyltrimethyl ammonium bromide (CTAB) in the polymerization mixture, the hybrid silica monolithic support was prepared in a 100 mu m i.d. capillary by the sol-gel method with tetraethoxysilane (TEOS) and 3-aminopropyltrietboxysilane (APTES) as precursors. Subsequently, the monolith was activated by glutaraldehyde, and trypsin was covalently immobilized. By monitoring the reaction of a decapeptide, C-myc (EQKLISEEDL), the enzymatic activity of the immobilized trypsin was calculated, and the results showed that the digestion speed was about 6600 times faster than that performed in free solution. The performance of such a microreactor was further demonstrated by digesting myoglobin, with the digested products analyzed by microflow reversed-phase liquid chromatography coupled with tandem mass spectrometry (mu RPLC-MS/MS). With a stringent threshold for the unambiguous identification of the digests, the yielding sequence coverage for on-column digestion was 92%, the same as that obtained by insolution: digestion, whereas the residence time of myoglobin in the former case was only 30 s, about 1/1440 Of that performed in the latter case (12 h). Moreover, such an immobilized trypsin reactor was also successfully applied to the digestion of a mixture of model proteins and proteins extracted from E. coli.
关键词[WOS]: ENABLING PROTEIN DIGESTION ;  MASS-SPECTROMETRY ;  CAPILLARY ELECTROCHROMATOGRAPHY ;  PROTEOLYTIC REACTOR ;  PEPTIDE SEPARATION ;  SOL-GEL ;  IDENTIFICATION ;  MICROREACTOR ;  ONLINE ;  SYSTEM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000254969100039
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100059
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Ma, Junfeng,Liang, Zhen,Qiao, Xiaoqiang,et al. Organic-inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity[J]. ANALYTICAL CHEMISTRY,2008,80(8):2949-2956.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Ma, Junfeng]'s Articles
 [Liang, Zhen]'s Articles
 [Qiao, Xiaoqiang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Ma, Junfeng]‘s Articles
 [Liang, Zhen]‘s Articles
 [Qiao, Xiaoqiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace