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题名: Induction of tobacco genes in response to oligochitosan
作者: Zhang, Fuyun;  Feng, Bin;  Li, Wei;  Bai, Xuefang;  Du, Yuguang;  Zhang, Yukui
通讯作者: 杜昱光
关键词: differential display ;  RT-PCR ;  oligochitosan ;  responsive gene
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2007-03-01
DOI: 10.1007/s11033-006-9008-8
卷: 34, 期:1, 页:35-40
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805,1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Oligochitosan has a variety of biological activities. To understand its mechanism, DDRT-PCR, reverse Northern blot and quantitative relative RT-PCR were used to identify and isolate genes whose transcription were altered in cultured Nicotiana tabacum (var. Samsun NN) plants that were treated with oligochitosan. Three genes whose mRNA levels significantly changed in response to oligochitosan were isolated and identified. One gene is up-regulated, and two genes are down-regulated. These genes encode a DNAJ heat shock N-terminal domain-containing protein, a histone H1 gene and a hypothetical protein, whose function is unknown. The results suggest that the usefulness of mRNA differential display technique for the detection of plant metabolic pathways affected by oligochitosan.
关键词[WOS]: RNA-POLYMERASE-II ;  HISTONE H1 ;  DIFFERENTIAL DISPLAY ;  N-ACETYLCHITOHEXAOSE ;  CHROMATIN-STRUCTURE ;  MOVEMENT PROTEIN ;  DNA METHYLATION ;  PLANT-PROTEINS ;  MESSENGER-RNA ;  TRANSCRIPTION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000244068100005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100115
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Fuyun,Feng, Bin,Li, Wei,et al. Induction of tobacco genes in response to oligochitosan[J]. MOLECULAR BIOLOGY REPORTS,2007,34(1):35-40.
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