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Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe
Tian, Ruijun1,2,3; Li, Lingdong2,3; Tang, Wei2,3; Liu, Hongwei1,2,3; Ye, Mingliang1,2; Zhao, Zongbao K.2; Zou, Hanfa1,2; Zou HF(邹汉法); Zou HF(邹汉法)
关键词2-d Nano Lc Chemical Probe Interactome Isoprenoid Chain Ms/ms Yeast Proteome
刊名PROTEOMICS
2008-08-01
ISSN1615-9853
DOI10.1002/pmic.200800021
8期:15页:3094-3104
收录类别SCI
文章类型Article
部门归属18
项目归属1809,1816
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]ACTIVATED PROTEIN-KINASE ; VIRUS-RNA REPLICATION ; FATTY-ACIDS ; SACCHAROMYCES-CEREVISIAE ; DIPHOSPHATE ANALOGS ; MEVALONATE KINASE ; MASS-SPECTROMETRY ; ALLOSTERIC SITES ; IDENTIFICATION ; PRENYLTRANSFERASES
英文摘要A chemical proteomic approach was developed for profiling the noncovalent interactome of isoprenoid chain in the yeast proteome. A chemical probe that harbors a biotin moiety and a photoreactive benzophenone group linked to the terminal of geranyl group was synthesized. Photoaffinity labeling was performed by incubating the Saccharomyces cerevisiae proteome and the probe under 365 nm. UV light. Thirty proteins were identified by immobilized NeutraAvidin enrichment, on-bead digestion, online 2-D nano-LC/MS/MS identification and semi-quantitative proteomic analysis. As noted by Gene Ontology annotation, the identified proteins demonstrate a wide range of catalytic activity in several biological processes, especially in metabolism and biosynthesis. Further data analysis shows that hydrophobic binding of the synthetic probe is potentially the major interaction force leading to covalent labeling. These results argue that intracellular allosteric interactions conferred by the isoprenoid chain of the corresponding chemical structures may be widespread at an interactomic level.
语种英语
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WOS记录号WOS:000258503400011
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/100133
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China
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Tian, Ruijun,Li, Lingdong,Tang, Wei,et al. Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe[J]. PROTEOMICS,2008,8(15):3094-3104.
APA Tian, Ruijun.,Li, Lingdong.,Tang, Wei.,Liu, Hongwei.,Ye, Mingliang.,...&邹汉法.(2008).Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe.PROTEOMICS,8(15),3094-3104.
MLA Tian, Ruijun,et al."Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe".PROTEOMICS 8.15(2008):3094-3104.
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