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题名: Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe
作者: Tian, Ruijun1, 2, 3;  Li, Lingdong2, 3;  Tang, Wei2, 3;  Liu, Hongwei1, 2, 3;  Ye, Mingliang1, 2;  Zhao, Zongbao K.2;  Zou, Hanfa1, 2
通讯作者: 邹汉法
关键词: 2-D nano LC ;  chemical probe ;  interactome ;  isoprenoid chain ;  MS/MS ;  yeast proteome
刊名: PROTEOMICS
发表日期: 2008-08-01
DOI: 10.1002/pmic.200800021
卷: 8, 期:15, 页:3094-3104
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809,1816
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: A chemical proteomic approach was developed for profiling the noncovalent interactome of isoprenoid chain in the yeast proteome. A chemical probe that harbors a biotin moiety and a photoreactive benzophenone group linked to the terminal of geranyl group was synthesized. Photoaffinity labeling was performed by incubating the Saccharomyces cerevisiae proteome and the probe under 365 nm. UV light. Thirty proteins were identified by immobilized NeutraAvidin enrichment, on-bead digestion, online 2-D nano-LC/MS/MS identification and semi-quantitative proteomic analysis. As noted by Gene Ontology annotation, the identified proteins demonstrate a wide range of catalytic activity in several biological processes, especially in metabolism and biosynthesis. Further data analysis shows that hydrophobic binding of the synthetic probe is potentially the major interaction force leading to covalent labeling. These results argue that intracellular allosteric interactions conferred by the isoprenoid chain of the corresponding chemical structures may be widespread at an interactomic level.
关键词[WOS]: ACTIVATED PROTEIN-KINASE ;  VIRUS-RNA REPLICATION ;  FATTY-ACIDS ;  SACCHAROMYCES-CEREVISIAE ;  DIPHOSPHATE ANALOGS ;  MEVALONATE KINASE ;  MASS-SPECTROMETRY ;  ALLOSTERIC SITES ;  IDENTIFICATION ;  PRENYLTRANSFERASES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000258503400011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100133
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Dalian 116023, Peoples R China

Recommended Citation:
Tian, Ruijun,Li, Lingdong,Tang, Wei,et al. Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe[J]. PROTEOMICS,2008,8(15):3094-3104.
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