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题名: The highly selective capture of phosphopeptides by zirconium phosphonate-modified magnetic nanoparticles for phosphoproteome analysis
作者: Zhao, Liang1;  Wu, Ren'an1;  Han, Guanghui1;  Zhou, Houjiang1;  Ren, Lianbing1;  Tian, Ruijun1;  Zou, Hanfa1
通讯作者: 邹汉法
刊名: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
发表日期: 2008-08-01
DOI: 10.1016/j.jasms.2008.04.027
卷: 19, 期:8, 页:1176-1186
收录类别: ISTP ;  SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Chemistry, Physical ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Spectroscopy
英文摘要: The highly selective capture of phosphopeptides from proteolytic digests is a great challenge for the identification of phosphoproteins by mass spectrometry. In this work, the zirconium phosphonate-modified magnetic Fe3O4/SiO2 core/shell nanoparticles have been synthesized and successfully applied for the selective capture of phosphopeptides From complex tryptic digests of proteins before the analysis of MALDI-TOF mass spectrometry with the desired convenience of sample handling. The ratio of magnetic nanoparticle to protein and the incubation time for capturing phosphopeptides from complex proteolytic digests were investigated, and the optimized nanoparticle-to-protein ratio and incubation time were between 15:1 to 30:1 and 30 min, respectively. The excellent detection limit of 0.5 fmol beta-casein has been achieved by MALDI-TOF mass spectrometry with the specific capture of zirconium phosphonate-modified magnetic Fe3O4 nanoparticles. The great specificity of zirconium phosphonate-modified magnetic Fe3O4 nanoparticles to phosphopeptides was demonstrated by the selective capture of phosphopeptides from a complex tryptic digest of the mixture of a-casein and bovine serum albumin at molar ratio of 1 to 100 in MALDI-TOF-MS analysis. An application of the magnetic nanoparticles to selective capture phosphopeptides from a tryptic digest of mouse liver lysate was further carried out by combining with nano-LC-MS/MS and MS/MS/MS analyses, and a total of 194 unique phosphopeptides were successfully identified.
关键词[WOS]: PROTEIN IDENTIFICATION TECHNOLOGY ;  FE(III) AFFINITY-CHROMATOGRAPHY ;  LARGE-SCALE ANALYSIS ;  MASS-SPECTROMETRY ;  PHOSPHORYLATED PROTEINS ;  PROTEOMIC ANALYSIS ;  MS ANALYSIS ;  ENRICHMENT ;  PEPTIDES ;  PARTICLES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000258538500018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100153
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Zhao, Liang,Wu, Ren'an,Han, Guanghui,et al. The highly selective capture of phosphopeptides by zirconium phosphonate-modified magnetic nanoparticles for phosphoproteome analysis[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2008,19(8):1176-1186.
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