中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Development of phosphopeptide enrichment techniques for phosphoproteome analysis
作者: Han, Guanghui1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
刊名: ANALYST
发表日期: 2008-09-01
DOI: 10.1039/b806775a
卷: 133, 期:9, 页:1128-1138
收录类别: SCI
文章类型: Review
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Protein phosphorylation is one of the most biologically relevant and ubiquitous post-translational modifications. The analysis of protein phosphorylation is very challenging due to its highly dynamic nature and low stoichiometry. In this article, recent techniques developed for phosphoproteome analysis are reviewed with an emphasis on the new developments in this field in China. To improve the performance of phosphoproteome analysis, many novel methods, either by application of new separation mechanisms or by adoption of new separation materials, were developed to specifically enrich phosphopeptides from complex protein digests. A series of new materials, including nanostructure materials, magnetic materials, and monolithic materials, were applied to prepare immobilized affinity chromatography or metal oxide affinity chromatography to improve the performance of phosphopeptide enrichment. Besides, new software tools were also developed to validate phosphopeptide identification and predict kinase specific phosphorylation sites.
关键词[WOS]: SITE-DIRECTED MUTAGENESIS ;  MALDI-TOF-MS ;  KINASE-SPECIFIC PHOSPHORYLATION ;  IONIZATION MASS-SPECTROMETRY ;  ION AFFINITY-CHROMATOGRAPHY ;  CORE-SHELL MICROSPHERES ;  HIGHLY SPECIFIC CAPTURE ;  MONOLITHIC CAPILLARY COLUMN ;  PHOSPHOAMINO ACID ANALYSIS ;  IMMOBILIZED METAL-IONS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000259449400002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100173
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Han, Guanghui,Ye, Mingliang,Zou, Hanfa. Development of phosphopeptide enrichment techniques for phosphoproteome analysis[J]. ANALYST,2008,133(9):1128-1138.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Han, Guanghui]'s Articles
 [Ye, Mingliang]'s Articles
 [Zou, Hanfa]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Han, Guanghui]‘s Articles
 [Ye, Mingliang]‘s Articles
 [Zou, Hanfa]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace