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Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter
Wang, Fangjun; Ye, Mingliang; Dong, Jing; Tian, Ruijun; Hu, Lianghai; Han, Guanghui; Jiang, Xinning; Wu, Ren'an; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Hydrophobic Monolith Label-free Quantitative Proteome Analysis Monolithic Esi Emitter Mouse Liver Nano-uplc-ms/mse
刊名JOURNAL OF SEPARATION SCIENCE
2008-08-01
ISSN1615-9306
DOI10.1002/jssc.200800181
31期:14页:2589-2597
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]SPECTROMETRY-BASED PROTEOMICS ; ORGANIC POLYMER MONOLITHS ; TANDEM MASS-SPECTROMETRY ; CODED AFFINITY TAGS ; ION-TRAP ; BIOMARKER DISCOVERY ; SHOTGUN PROTEOMICS ; STATIONARY PHASES ; QUANTIFICATION ; PROTEINS
英文摘要The postcolumn void volume, which is introduced by the connecting tubing and void ESI emitter in the nanoflow LC coupled with MS/MS system (mu LC-MS/MS), is harmful for the analysis of peptides in shotgun proteome analysis. A new type of porous C12 monolithic ESI emitter was prepared to eliminate the disruption and mixing effects occurring in the connecting tubing and void emitter. It was demonstrated that the porous hydrophobic monolith inside the emitter played a key role in retaining the good peak profile, and the average peak capacity of the whole separation system increased 12.8% in contrast to commercially available void emitter. Then, the porous Cl 2 monolithic emitter was applied in label-free quantitative proteome analysis of two standard protein mixtures that were spiked into the tryptic digest of mouse livers extract. Compared to commercially available void ESI emitter, the number of proteins with reliable results in quantification increased greatly. And the relative quantities of the four standard proteins were all determined With the relative error <= 6.8%. However, quantitative information of only three standard proteins could be obtained when void emitter was used.
语种英语
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WOS记录号WOS:000258668000008
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/100175
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Wang, Fangjun,Ye, Mingliang,Dong, Jing,et al. Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(14):2589-2597.
APA Wang, Fangjun.,Ye, Mingliang.,Dong, Jing.,Tian, Ruijun.,Hu, Lianghai.,...&邹汉法.(2008).Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter.JOURNAL OF SEPARATION SCIENCE,31(14),2589-2597.
MLA Wang, Fangjun,et al."Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter".JOURNAL OF SEPARATION SCIENCE 31.14(2008):2589-2597.
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