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Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter
Wang, Fangjun; Ye, Mingliang; Dong, Jing; Tian, Ruijun; Hu, Lianghai; Han, Guanghui; Jiang, Xinning; Wu, Ren'an; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordHydrophobic Monolith Label-free Quantitative Proteome Analysis Monolithic Esi Emitter Mouse Liver Nano-uplc-ms/mse
Source PublicationJOURNAL OF SEPARATION SCIENCE
2008-08-01
ISSN1615-9306
DOI10.1002/jssc.200800181
Volume31Issue:14Pages:2589-2597
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSPECTROMETRY-BASED PROTEOMICS ; ORGANIC POLYMER MONOLITHS ; TANDEM MASS-SPECTROMETRY ; CODED AFFINITY TAGS ; ION-TRAP ; BIOMARKER DISCOVERY ; SHOTGUN PROTEOMICS ; STATIONARY PHASES ; QUANTIFICATION ; PROTEINS
AbstractThe postcolumn void volume, which is introduced by the connecting tubing and void ESI emitter in the nanoflow LC coupled with MS/MS system (mu LC-MS/MS), is harmful for the analysis of peptides in shotgun proteome analysis. A new type of porous C12 monolithic ESI emitter was prepared to eliminate the disruption and mixing effects occurring in the connecting tubing and void emitter. It was demonstrated that the porous hydrophobic monolith inside the emitter played a key role in retaining the good peak profile, and the average peak capacity of the whole separation system increased 12.8% in contrast to commercially available void emitter. Then, the porous Cl 2 monolithic emitter was applied in label-free quantitative proteome analysis of two standard protein mixtures that were spiked into the tryptic digest of mouse livers extract. Compared to commercially available void ESI emitter, the number of proteins with reliable results in quantification increased greatly. And the relative quantities of the four standard proteins were all determined With the relative error <= 6.8%. However, quantitative information of only three standard proteins could be obtained when void emitter was used.
Language英语
URL查看原文
WOS IDWOS:000258668000008
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/100175
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, Fangjun,Ye, Mingliang,Dong, Jing,et al. Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(14):2589-2597.
APA Wang, Fangjun.,Ye, Mingliang.,Dong, Jing.,Tian, Ruijun.,Hu, Lianghai.,...&邹汉法.(2008).Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter.JOURNAL OF SEPARATION SCIENCE,31(14),2589-2597.
MLA Wang, Fangjun,et al."Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter".JOURNAL OF SEPARATION SCIENCE 31.14(2008):2589-2597.
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