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题名: Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter
作者: Wang, Fangjun1;  Ye, Mingliang1;  Dong, Jing1;  Tian, Ruijun1;  Hu, Lianghai1;  Han, Guanghui1;  Jiang, Xinning1;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: hydrophobic monolith ;  label-free quantitative proteome analysis ;  monolithic ESI emitter ;  mouse liver ;  nano-UPLC-MS/MSE
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2008-08-01
DOI: 10.1002/jssc.200800181
卷: 31, 期:14, 页:2589-2597
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The postcolumn void volume, which is introduced by the connecting tubing and void ESI emitter in the nanoflow LC coupled with MS/MS system (mu LC-MS/MS), is harmful for the analysis of peptides in shotgun proteome analysis. A new type of porous C12 monolithic ESI emitter was prepared to eliminate the disruption and mixing effects occurring in the connecting tubing and void emitter. It was demonstrated that the porous hydrophobic monolith inside the emitter played a key role in retaining the good peak profile, and the average peak capacity of the whole separation system increased 12.8% in contrast to commercially available void emitter. Then, the porous Cl 2 monolithic emitter was applied in label-free quantitative proteome analysis of two standard protein mixtures that were spiked into the tryptic digest of mouse livers extract. Compared to commercially available void ESI emitter, the number of proteins with reliable results in quantification increased greatly. And the relative quantities of the four standard proteins were all determined With the relative error <= 6.8%. However, quantitative information of only three standard proteins could be obtained when void emitter was used.
关键词[WOS]: SPECTROMETRY-BASED PROTEOMICS ;  ORGANIC POLYMER MONOLITHS ;  TANDEM MASS-SPECTROMETRY ;  CODED AFFINITY TAGS ;  ION-TRAP ;  BIOMARKER DISCOVERY ;  SHOTGUN PROTEOMICS ;  STATIONARY PHASES ;  QUANTIFICATION ;  PROTEINS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000258668000008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100175
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Fangjun,Ye, Mingliang,Dong, Jing,et al. Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(14):2589-2597.
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