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Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis
Zhou, Houjiang; Ye, Mingliang; Dong, Jing; Han, Guanghui; Jiang, Xinning; Wu, Renan; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Immobilized Metal Ion Affinity Chromatography Titanium Ion Phosphate Polymer Phosphopeptide Enrichment Phosphoproteome Analysis
刊名JOURNAL OF PROTEOME RESEARCH
2008-09-01
ISSN1535-3893
DOI10.1021/pr800223m
7期:9页:3957-3967
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]TANDEM MASS-SPECTROMETRY ; SCALE PHOSPHORYLATION ANALYSIS ; HIGHLY SELECTIVE ENRICHMENT ; NANO-LC-MS/MS ; SACCHAROMYCES-CEREVISIAE ; MAGNETIC NANOPARTICLES ; METHYL ESTERIFICATION ; PEPTIDE MIXTURES ; MOUSE-LIVER ; MS ANALYSIS
英文摘要The elucidation of protein post-translational modifications, such as phosphorylation, remains a challenging analytical task for proteomic studies. Since many of the proteins targeted for phosphorylation are low in abundance and phosphorylation is typically substoichiometric, a prerequisite for their identification is the specific enrichment of phosphopeptide prior to mass spectrometric analysis. Here, we presented a new method termed as immobilized titanium ion affinity chromatography (Ti4+-IMAC) for enriching phosphopeptides. A phosphate polymer, which was prepared by direct polymerization of monomers containing phosphate groups, was applied to immobilize Ti4+ through the chelating interaction between phosphate groups on the polymer and Ti4+. The resulting Ti4+-IMAC resin specifically isolates phosphopeptides from a digest mixture of standard phosphoproteins and nonphosphoprotein (BSA) in a ratio as low as 1:500. Ti4+-IMAC was further applied for phosphoproteome analysis of mouse liver. We also compared Ti4+-IMAC to other enrichment methods including Fe3+-IMAC, Zr4+-IMAC, TiO2 and ZrCl2 and demonstrate superior selectivity and efficiency of Ti4+-IMAC for the isolation and enrichment of phosphopeptides. The high specificity and efficiency of phosphopeptide enrichment by Ti4+-IMAC mainly resulted from the flexibility of immobilized titanium ion with spacer arm linked to polymer beads as well as the specific interaction between immobilized titanium ion and phosphate group on phosphopeptides.
语种英语
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WOS记录号WOS:000259015500031
引用统计
被引频次:190[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/100199
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Zhou, Houjiang,Ye, Mingliang,Dong, Jing,et al. Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis[J]. JOURNAL OF PROTEOME RESEARCH,2008,7(9):3957-3967.
APA Zhou, Houjiang.,Ye, Mingliang.,Dong, Jing.,Han, Guanghui.,Jiang, Xinning.,...&邹汉法.(2008).Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis.JOURNAL OF PROTEOME RESEARCH,7(9),3957-3967.
MLA Zhou, Houjiang,et al."Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis".JOURNAL OF PROTEOME RESEARCH 7.9(2008):3957-3967.
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