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题名: Metabolic response of different osmo-sensitive Sacchromyces cerevisiae to ACA microcapsule
作者: Zhi-jie, Sun1, 3;  Shuang-yue, Li1, 3;  Guo-jun, Lv1, 3;  Jing, Zhu1;  Wei-ting, Yu1;  Wei, Wang1;  Xiaojun, Ma1, 2
通讯作者: 马小军
关键词: Sacchromyces cerevisiae ;  osmo-sensitive ;  ACA microcapsules ;  metabolism
刊名: ENZYME AND MICROBIAL TECHNOLOGY
发表日期: 2008-06-05
DOI: 10.1016/j.enzmictec.2008.01.021
卷: 42, 期:7, 页:576-582
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Microencapsulation technology is a convenient method to alter and regulate cell product formation. In order to probe the metabolic response of different osmo-sensitive Sacchromyces cerevisiae to ACA microcapsule, the hyper-osmo-sensitive type S. cerevisiae (Y02724) and wild type S. cerevisiae (BY4741) were encapsulated into liquid core ACA microcapsules. The behavior of cell growth, glucose consumption, ethanol production and the yields of glycerol and organic acids were determined. Free cell culture was used as control. The enzyme activities of NADP(+)-glutamate dehydrogenase (GDH), glutamine synthetase (GS) and glutamate synthase (GOGAT) on microencapsulation cells and free cultured cells were measured too. The results demonstrated that the growth of Y02724 in both aerobic and anaerobic conditions was seriously inhibited by ACA microcapsule, while the ethanol and acetatic acid yield of microencapsulation Y02724 in anaerobic condition were significantly higher than that of suspended cultivation. For Y02724, the microencapsulation cultivation significantly increased the GS and GOGAT activities and decreased the GDH activity in comparison with control group. ACA microcapsules did not significantly change the growth behavior and metabolic performance of BY4741, but decreased the GS activity. In conclusion, microcapsules microenvironment significantly changes the metabolism behavior of hyper-osmo-sensitive type S. cerevisiae (Y02724), but nearly had no effect on BY4741. (c) 2008 Elsevier Inc. All rights reserved.
关键词[WOS]: PERTURBING COMPOUND CHITOSAN ;  SACCHAROMYCES-CEREVISIAE ;  GLUCOSE-METABOLISM ;  CELLS ;  YEAST ;  IMMOBILIZATION ;  STRESS ;  MICROENCAPSULATION ;  MICROENVIRONMENT ;  ENCAPSULATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000256640400007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100257
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhi-jie, Sun,Shuang-yue, Li,Guo-jun, Lv,et al. Metabolic response of different osmo-sensitive Sacchromyces cerevisiae to ACA microcapsule[J]. ENZYME AND MICROBIAL TECHNOLOGY,2008,42(7):576-582.
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