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题名: Transplantation of microencapsulated genetically modified xenogeneic cells augments angiogenesis and improves heart function
作者: Zhang, H.1, 2, 3;  Zhu, S-J3;  Wang, W.4;  Wei, Y-J3;  Hu, S-S1, 2, 3
通讯作者: S-S Hu
关键词: microencapsulation ;  myocardial infarction ;  angiogenesis ;  vascular endothelial growth factor
刊名: GENE THERAPY
发表日期: 2008
DOI: 10.1038/sj.gt.3303049
卷: 15, 期:1, 页:40-48
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 3;3
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Genetics & Heredity ;  Medicine, Research & Experimental
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Genetics & Heredity ;  Research & Experimental Medicine
英文摘要: Cell-based gene therapy offers an alternative strategy for therapeutic angiogenesis for the management of myocardial infarction (MI). However, immune rejection poses a significant obstacle to the implantation of genetically engineered allogeneic or xenogeneic cells. In the present study, an ex vivo gene therapy approach utilizing cell microencapsulation was employed to deliver vascular endothelial growth factor ( VEGF) to ischemic myocardium. Chinese hamster ovary (CHO) cells were genetically modified to secrete VEGF and enveloped into semipermeable microcapsules. In vitro assay indicated that the microencapsulated engineered CHO cells could secrete VEGF as high as 3852 pg ml (1) per 48 h at day 8 after encapsulation. Then the microencapsulated CHO cells were implanted into the injured myocardium in a rat MI model, while engineered CHO cells, blank microcapsules and serum-free culture media were implanted as controls. The humoral immunity to xenogeneic CHO cells were evaluated and we found that the titer of anti-CHO antibodies was significantly lower in the microencapsulated CHO transplantation group than the group receiving unencapsulated CHO cells at two weeks after implantation. However, 1 week later, there was almost no difference between these groups. Histology and western blotting confirmed that the microencapsulated CHO cells maintained their original structure and VEGF secretion three weeks after implantation. The capillary density in the treatment region was also significantly higher in the microencapsulated CHO cell group than control groups, which was consistent with gross heart functional improvement. These data suggest that microencapsulated xenogeneic cell-based gene therapy might be a novel approach for therapeutic angiogenesis in ischemic heart disease.
关键词[WOS]: ALGINATE-BASED MICROCAPSULES ;  POLY-L-LYSINE ;  THERAPEUTIC ANGIOGENESIS ;  GENE-THERAPY ;  MYOCARDIUM ;  DISEASE ;  ISLETS ;  RATS ;  IMMUNOSUPPRESSION ;  VASCULOGENESIS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000251694800005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100261
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Cardiovasc Inst, Dept Cardiac Surg, Beijing 100037, Peoples R China
2.Chinese Acad Med Sci, Fu Wai Hosp, Beijing 100037, Peoples R China
3.Minist Hlth, Res Ctr Cardiac Regenerat Med, Beijing, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian, Peoples R China

Recommended Citation:
Zhang, H.,Zhu, S-J,Wang, W.,et al. Transplantation of microencapsulated genetically modified xenogeneic cells augments angiogenesis and improves heart function[J]. GENE THERAPY,2008,15(1):40-48.
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