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Vacuum-evaporation interface of comprehensive two-dimensional liquid chromatography and its application
Tian Hong-Zhe1,2; Xu Jing2; Guan Ya-Feng2; Guan YF(关亚风); Guan YF(关亚风)
KeywordComperhensive Two-dimensional Liquid Chromatography Normal Phase Liquid Chromatography-reversed Phae Liquid Chromatography (Nplc-aplc) Vacuum-evaporation Interface Cnidium Monnieri(L.) Cuss
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2008-06-01
Volume36Issue:6Pages:860-864
Indexed BySCI
SubtypeArticle
Department1
Funding Project105
Contribution Rank2;1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSEPARATIONS
AbstractA comprehensive two-dimensional liquid chromatographic system (NPLC-RPLC) was developed using a vacuum-evaporation interface (VEI). A normal-phase liquid chromatography (NPLC) with a CN microcolumn was used as the first dimension (1(st)-D), and a reversed-phase liquid chromatography (RPLC) with a C-18 monolithic column was used as the second dimension (2(nd)-D). A two-position, ten-port valve with two identical storage loops was used as the interface that enabled alternate sampling of the first column eluent onto the second column untill the analysis was finished. In one of the two positions, the eluent from the first column filled one of the loops and was evaporated at 25 degrees C under vacuum conditions, leaving the analytes stick on to the inner wall of the loop. When the valve was switched to the second position, the effluent from the first column flowed through the other loop, while mobile phase of the 2(nd)-D dissolved the analytes in the loop from the previous cycle and injected them onto the second column, allowing on-line solvent exchange of the fractions from the 1(st)-D to the 2(nd)-D. The utility of the comprehensive 2DLC system was demonstrated in the analysis of standard samples and traditional Chinese medicine Cnidium monnieri (L.) Cuss extract.
Language英语
URL查看原文
WOS IDWOS:000257877300030
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/100317
Collection中国科学院大连化学物理研究所
Corresponding AuthorGuan YF(关亚风); Guan YF(关亚风)
Affiliation1.Shenyang Agr Univ, Analyt Ctr, Shenyang 110161, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Tian Hong-Zhe,Xu Jing,Guan Ya-Feng,et al. Vacuum-evaporation interface of comprehensive two-dimensional liquid chromatography and its application[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(6):860-864.
APA Tian Hong-Zhe,Xu Jing,Guan Ya-Feng,关亚风,&关亚风.(2008).Vacuum-evaporation interface of comprehensive two-dimensional liquid chromatography and its application.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(6),860-864.
MLA Tian Hong-Zhe,et al."Vacuum-evaporation interface of comprehensive two-dimensional liquid chromatography and its application".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.6(2008):860-864.
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