中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry
作者: Gu, Qun1;  Shi, Xianzhe1;  Yin, Peiyuan1;  Gao, Peng1;  Lu, Xin1;  Xu, Gumang1
通讯作者: 许国旺
关键词: catecholamine ;  metabolite ;  liquid chromatography ;  tandem mass spectrometry ;  adrenal gland
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2008-02-25
DOI: 10.1016/j.aca.2008.01.017
卷: 609, 期:2, 页:192-200
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Two simple, rapid and specific analytical methods for 13 catecholamines and their metabolites have been developed based on liquid chromatography tandem mass spectrometry in a multiple reaction monitoring mode. Tyrosine, dopamine, dihydroxyphenylalanine, epinephrine, norepinephrine, 3-methoxytyramine, normetanephrine, metanephrine and isoproterenol (internal standard) were separated on a Kromasil (TM) Cyano, analytical column by a mobile phase consisting of 60% (v/v) acetonitrile and 40% (v/v) water adjusted with formic acid to pH 3.0, and detected by positive ionization electrospray tandem mass spectrometry. While vanillymandelic acid, 3,4-dihydroxymandelic acid, homovanillic acid, 3,4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylglycol and 5-hydroxy-2-indolecarboxylic acid (internal standard) were separated on a reversed-phase Shim-Pak VP-ODS column with the mobile phase of 60% (v/v) acetonitrile, and 40% (v/v) water adjusted with formic acid to pH 4.5 and detected in the negative ionization electrospray tandem mass spectrometry. The influence of various parameters such as column type and mobile phase composition on separation and sensitivity were investigated. The limits of detection were in the range of 0.5-20 ng mL(-1). The mean recoveries determined from three different concentrations of each analyte were above 85.4%. The precision of the method calculated as relative standard deviation was lower than 5.3%. Deduced from the results of real sample analysis, adrenal gland synthesizes and stores the catecholamine hormones norepinephrine and epinephrine. (c) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: PLASMA-FREE METANEPHRINE ;  SOLID-PHASE EXTRACTION ;  CAPILLARY-ELECTROPHORESIS ;  ELECTROCHEMICAL DETECTION ;  FLUORESCENCE DETECTION ;  L-DOPA ;  NEUROTRANSMITTERS ;  EPINEPHRINE ;  URINE ;  NOREPINEPHRINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000253616500007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100427
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Gu, Qun,Shi, Xianzhe,Yin, Peiyuan,et al. Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2008,609(2):192-200.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Gu, Qun]'s Articles
 [Shi, Xianzhe]'s Articles
 [Yin, Peiyuan]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Gu, Qun]‘s Articles
 [Shi, Xianzhe]‘s Articles
 [Yin, Peiyuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace