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Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry
Gu, Qun; Shi, Xianzhe; Yin, Peiyuan; Gao, Peng; Lu, Xin; Xu, Gumang; Xu GW(许国旺)
关键词Catecholamine Metabolite Liquid Chromatography Tandem Mass Spectrometry Adrenal Gland
刊名ANALYTICA CHIMICA ACTA
2008-02-25
DOI10.1016/j.aca.2008.01.017
609期:2页:192-200
收录类别SCI
文章类型Article
部门归属18
项目归属1808
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PLASMA-FREE METANEPHRINE ; SOLID-PHASE EXTRACTION ; CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; FLUORESCENCE DETECTION ; L-DOPA ; NEUROTRANSMITTERS ; EPINEPHRINE ; URINE ; NOREPINEPHRINE
英文摘要Two simple, rapid and specific analytical methods for 13 catecholamines and their metabolites have been developed based on liquid chromatography tandem mass spectrometry in a multiple reaction monitoring mode. Tyrosine, dopamine, dihydroxyphenylalanine, epinephrine, norepinephrine, 3-methoxytyramine, normetanephrine, metanephrine and isoproterenol (internal standard) were separated on a Kromasil (TM) Cyano, analytical column by a mobile phase consisting of 60% (v/v) acetonitrile and 40% (v/v) water adjusted with formic acid to pH 3.0, and detected by positive ionization electrospray tandem mass spectrometry. While vanillymandelic acid, 3,4-dihydroxymandelic acid, homovanillic acid, 3,4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylglycol and 5-hydroxy-2-indolecarboxylic acid (internal standard) were separated on a reversed-phase Shim-Pak VP-ODS column with the mobile phase of 60% (v/v) acetonitrile, and 40% (v/v) water adjusted with formic acid to pH 4.5 and detected in the negative ionization electrospray tandem mass spectrometry. The influence of various parameters such as column type and mobile phase composition on separation and sensitivity were investigated. The limits of detection were in the range of 0.5-20 ng mL(-1). The mean recoveries determined from three different concentrations of each analyte were above 85.4%. The precision of the method calculated as relative standard deviation was lower than 5.3%. Deduced from the results of real sample analysis, adrenal gland synthesizes and stores the catecholamine hormones norepinephrine and epinephrine. (c) 2008 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000253616500007
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被引频次:56[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/100427
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Gu, Qun,Shi, Xianzhe,Yin, Peiyuan,et al. Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2008,609(2):192-200.
APA Gu, Qun.,Shi, Xianzhe.,Yin, Peiyuan.,Gao, Peng.,Lu, Xin.,...&许国旺.(2008).Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry.ANALYTICA CHIMICA ACTA,609(2),192-200.
MLA Gu, Qun,et al."Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry".ANALYTICA CHIMICA ACTA 609.2(2008):192-200.
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