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题名: Development of a comprehensive two-dimensional hydrophilic interaction chromatography/quadrupole time-of-flight mass spectrometry system and its application in separation and identification of saponins from Quillaja saponaria
作者: Wang, Yuan1;  Lu, Xin1;  Xu, Guowang1
通讯作者: 许国旺
关键词: HILIC ;  comprehensive two-dimensional liquid chromatography ;  orthogonality ;  quillaja saponins
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2008-02-15
DOI: 10.1016/j.chroma.2007.12.034
卷: 1181, 期:1-2, 页:51-59
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Hydrophilic interaction chromatography (HILIC) was used in two dimensions in a comprehensive two-dimensional HILIC hyphenated with a quadrupole time-of-flight mass spectrometry (HILIC x HILIC-Q-TOF-MS) system for the analysis of complex samples of hydrophilic compounds. A TSKgel Amide-80 column was employed as the first dimension, and a short PolyHydroxyethyl A column was as the second dimension. The column system showed moderate orthogonality at defined operational conditions. A high speed Q-TOF-MS detector as a third complementary dimension significantly improved the peak capacity. The separation capability of the developed HILIC x HILIC-Q-TOF-MS system was tested by separating an extract from Quillaja saponaria. The major components, quillaja saponins, in the extract were well identified by means of [M-H](-) ions, characteristic product ions, and their two-dimensional retention behaviors. Several pairs of isomers, which were often co-eluted on conventional LC-MS methods and had similar fragmentation characteristics in MS/MS spectra, were well separated on the two-dimensional system based on their different hydrophilicity. The developed comprehensive two-dimensional HILIC system demonstrates unique selectivity for hydrophilic compounds and satisfactory peak capacity and resolution for analogues by making sufficient use of two-dimensional separation plane. (c) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: PHASE LIQUID-CHROMATOGRAPHY ;  PEPTIDE SEPARATION ;  SAMPLE PREPARATION ;  STATIONARY PHASES ;  ONLINE ;  HPLC ;  ORTHOGONALITY ;  COLUMN ;  TRIACYLGLYCEROLS ;  SELECTIVITY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000253506600006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100429
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Yuan,Lu, Xin,Xu, Guowang. Development of a comprehensive two-dimensional hydrophilic interaction chromatography/quadrupole time-of-flight mass spectrometry system and its application in separation and identification of saponins from Quillaja saponaria[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1181(1-2):51-59.
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