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题名: Study on the effect of eicosapentaenoic acid on phospholipids composition in membrane microdomains of tight junctions of epithelial cells by liquid chromatography/electrospray mass spectrometry
作者: Zhao, Sumin1;  Jia, Lewen1;  Gao, Peng1;  Li, Qiurong2;  Lu, Xin1;  Li, Jieshou2;  Xu, Guowang1
通讯作者: 许国旺
关键词: eicosapentaenoic acid ;  tight junction ;  phospholipids ;  HPLC-MS
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2008-06-09
DOI: 10.1016/j.jpba.2008.01.005
卷: 47, 期:2, 页:343-350
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: Tight junctions of epithelial cells determine epithelial membrane integrity and play an important role in selective paracellular permeability to ions and macromolecules. In this work, we investigated the effect of one of n-3 series polyunsaturated fatty acids, eicosapentaenoic acid (EPA) on the phospholipid composition of membrane microdomains of tight junctions. After treated by EPA, membrane microdomains of tight junctions were isolated by discontinuous sucrose density gradient ultracentrifugation, and raft phospholipids were extracted. The PE, PI, PS, PC and SM were separated and determined by high-performance liquid chromatography/quadrupole-linear ion trap mass spectrometry (HPLC Qtrap-MS), and were further identified by HPLC-MS/MS. It was found that EPA altered the fatty acyl substitution of phospholipids that constituted membrane microdomains of tight junctions by enriching the unsaturated fatty acyl chains of the phospholipids. It provides a new visual angle to explaining the intracellular mechanism involved in n-3 polyunsaturated fatty acids (PUFAs) modulation of intestinal tight junction barrier. (c) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: POLYUNSATURATED FATTY-ACIDS ;  CROHNS-DISEASE ;  HUMAN BLOOD ;  INTESTINAL PERMEABILITY ;  CACO-2 CELLS ;  IDENTIFICATION ;  PROTEINS ;  BARRIER
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000256649400016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100437
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China
2.Jinling Hosp, Inst Gen Surg, Nanjing 210002, Peoples R China

Recommended Citation:
Zhao, Sumin,Jia, Lewen,Gao, Peng,et al. Study on the effect of eicosapentaenoic acid on phospholipids composition in membrane microdomains of tight junctions of epithelial cells by liquid chromatography/electrospray mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2008,47(2):343-350.
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