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题名: Simultaneous separation of hydrophilic and hydrophobic compounds by using an online HILIC-RPLC system with two detectors
作者: Wang, Yuan1;  Lu, Xin1;  Xu, Guowang1
通讯作者: 许国旺
关键词: HILIC ;  orthogonality ;  traditional Chinese medicine ;  two-dimensional liquid chromatography
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2008-05-01
DOI: 10.1002/jssc.200700663
卷: 31, 期:9, 页:1564-1572
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: In the present study, we developed a novel online hydrophilic interaction chromatography (HILIC)-RPLC separation system for simultaneous separation of both hydrophilic and hydrophobic solutes in a complex sample with one injection. A HILIC Si column and a C18 column were hyphenated with an interface including two electronic 2-position valves, a solvent pump, and a solute transfer column. By using column-switching technique, the nonretained hydrophobic solutes were eluted out of the HILIC column and transferred into the C18 column to perform further separation. The hydrophilic solutes were separated on the HILIC column at the same time. One detector was equipped for each column to record the individual chromatograms. By separating a standard mixture and a traditional Chinese medicine (TCM) extract, the developed HILIC-RPLC system demonstrates its potential for "entire-components" separation of complex samples with improved peak capacity and throughput compared with the common single-column LC.
关键词[WOS]: INTERACTION LIQUID-CHROMATOGRAPHY ;  TANDEM MASS-SPECTROMETRY ;  LIGHT-SCATTERING DETECTION ;  HUMAN PLASMA ;  PEPTIDE SEPARATION ;  STATIONARY-PHASE ;  POLAR COMPOUNDS ;  COLUMNS ;  GLUTATHIONE ;  ACID
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000256535800014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100441
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Yuan,Lu, Xin,Xu, Guowang. Simultaneous separation of hydrophilic and hydrophobic compounds by using an online HILIC-RPLC system with two detectors[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(9):1564-1572.
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