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题名: Determination of organic acids in the presence of inorganic anions by ion chromatography with suppressed conductivity detection
作者: Geng, Xiumei1;  Zhang, Sufang1;  Wang, Qian1;  Zhao, Zongbao (Kent)1
通讯作者: 赵宗保
关键词: ion chromatography ;  conductivity detection ;  organic acids ;  culture media ;  peptone ;  yeast extract
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2008-05-23
DOI: 10.1016/j.chroma.2008.03.073
卷: 1192, 期:1, 页:187-190
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Simultaneous separation of 19 organic acids and 10 inorganic anions has been demonstrated using ion chromatography with a high capacity anion exchange column and the suppressed conductivity detector under an auto-suppression external sulfuric acid mode. Quantitative merits of this method were examined for analysis of nine organic acids of potential significance in cell culture broth. External calibration curves for these analytes were linear with correlation coefficients exceeding 0.999, and the relative standard derivations of observed analyte concentrations were less than 3.0% in both inter- and intra-day evaluations of aqueous standards. Developed methodology was subsequently applied to obtain organic acid profiles of Luria-Bertani liquid media, yeast extract, peptone, and the culture broth of a mutant Escherichia coli strain. Analytes recoveries observed for triplicate analysis of LB media spiked at two concentration levels ranged from 88% to 105% with less than 7% RSD. These data demonstrate quantitative accuracy for LB media and suggest that the report method may also be applicable to complex samples such as fermentation mixture and lignocellulose hydrolysate. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TANDEM MASS-SPECTROMETRY ;  CARBOXYLIC-ACIDS ;  FERMENTATION ;  WATER ;  COLI
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000256200700024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100549
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Geng, Xiumei,Zhang, Sufang,Wang, Qian,et al. Determination of organic acids in the presence of inorganic anions by ion chromatography with suppressed conductivity detection[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1192(1):187-190.
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