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Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors
Lu, Xianbo; Zhang, Haijun; Ni, Yuwen; Zhang, Qing; Chen, Jiping; Chen JP(陈吉平); Chen JP(陈吉平)
关键词Biosensor Direct Electron Transfer Hydrogen Peroxide Nitrite Zno
刊名BIOSENSORS & BIOELECTRONICS
2008-09-15
DOI10.1016/j.bios.2008.03.025
24期:1页:93-98
收录类别SCI
文章类型Article
部门归属1
项目归属103
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]HYDROGEN-PEROXIDE BIOSENSOR ; DIRECT ELECTRON-TRANSFER ; HEME-PROTEINS ; CARBON NANOTUBE ; ZINC-OXIDE ; HEMOGLOBIN ; CHITOSAN ; FILMS ; ELECTROCATALYSIS ; IMMOBILIZATION
英文摘要Nanosheet-based ZnO microsphere with porous nanostructures was synthesized by a facile chemical bath deposition method followed by thermal treatment. which was explored for the construction of electrochemical biosensors. Spectroscopic and electrochemical researches revealed the ZnO-based composite was a biocompatible immobilization matrix for enzymes with good enzymatic stability and bioactivity. With advantages of nanostructured inorganic-organic hybrid materials, a pair of stable and well-defined quasi-reversible redox peaks of hemoglobin was obtained with a formal potential of -0.345 V (vs. Ag/AgCl) in pH 7.0 buffer. Facilitated direct electron transfer of the metalloenzymes with an apparent heterogeneous electron transfer rate constant (k(s)) of 3.2 s(-1) was achieved on the ZnO-based enzyme electrode. Comparative studies demonstrated the nanosheet-based ZnO microspheres were more effective in facilitating the electron transfer of immobilized enzyme than solid ZnO microspheres, which may result from the unique nanostructures and larger surface area of the porous ZnO. The prepared biosensor displayed good performance for the detection of H2O2 and NaNO2 with a wide linear range of 1-410 and 10-2700 mu M, respectively. The entrapped hemoglobin exhibits high peroxidase-like activity for the catalytic reduction Of H2O2 with an apparent Michaelis-Menten constant (K-M(app)) of 143 mu M. The nanosheet-based ZnO could be a promising matrix for the fabrication of direct electrochemical biosensors, and may find wide potential applications in biomedical detection and environmental analysis. (C) 2008 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000259425300014
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被引频次:138[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/100687
专题中国科学院大连化学物理研究所
通讯作者Chen JP(陈吉平); Chen JP(陈吉平)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Lu, Xianbo,Zhang, Haijun,Ni, Yuwen,et al. Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors[J]. BIOSENSORS & BIOELECTRONICS,2008,24(1):93-98.
APA Lu, Xianbo.,Zhang, Haijun.,Ni, Yuwen.,Zhang, Qing.,Chen, Jiping.,...&陈吉平.(2008).Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors.BIOSENSORS & BIOELECTRONICS,24(1),93-98.
MLA Lu, Xianbo,et al."Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors".BIOSENSORS & BIOELECTRONICS 24.1(2008):93-98.
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