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题名: Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors
作者: Lu, Xianbo1;  Zhang, Haijun1;  Ni, Yuwen1;  Zhang, Qing1;  Chen, Jiping1
通讯作者: 陈吉平
关键词: biosensor ;  direct electron transfer ;  hydrogen peroxide ;  nitrite ;  ZnO
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2008-09-15
DOI: 10.1016/j.bios.2008.03.025
卷: 24, 期:1, 页:93-98
收录类别: SCI
文章类型: Article
部门归属: 1
项目归属: 103
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: Nanosheet-based ZnO microsphere with porous nanostructures was synthesized by a facile chemical bath deposition method followed by thermal treatment. which was explored for the construction of electrochemical biosensors. Spectroscopic and electrochemical researches revealed the ZnO-based composite was a biocompatible immobilization matrix for enzymes with good enzymatic stability and bioactivity. With advantages of nanostructured inorganic-organic hybrid materials, a pair of stable and well-defined quasi-reversible redox peaks of hemoglobin was obtained with a formal potential of -0.345 V (vs. Ag/AgCl) in pH 7.0 buffer. Facilitated direct electron transfer of the metalloenzymes with an apparent heterogeneous electron transfer rate constant (k(s)) of 3.2 s(-1) was achieved on the ZnO-based enzyme electrode. Comparative studies demonstrated the nanosheet-based ZnO microspheres were more effective in facilitating the electron transfer of immobilized enzyme than solid ZnO microspheres, which may result from the unique nanostructures and larger surface area of the porous ZnO. The prepared biosensor displayed good performance for the detection of H2O2 and NaNO2 with a wide linear range of 1-410 and 10-2700 mu M, respectively. The entrapped hemoglobin exhibits high peroxidase-like activity for the catalytic reduction Of H2O2 with an apparent Michaelis-Menten constant (K-M(app)) of 143 mu M. The nanosheet-based ZnO could be a promising matrix for the fabrication of direct electrochemical biosensors, and may find wide potential applications in biomedical detection and environmental analysis. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROGEN-PEROXIDE BIOSENSOR ;  DIRECT ELECTRON-TRANSFER ;  HEME-PROTEINS ;  CARBON NANOTUBE ;  ZINC-OXIDE ;  HEMOGLOBIN ;  CHITOSAN ;  FILMS ;  ELECTROCATALYSIS ;  IMMOBILIZATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000259425300014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100687
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Lu, Xianbo,Zhang, Haijun,Ni, Yuwen,et al. Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors[J]. BIOSENSORS & BIOELECTRONICS,2008,24(1):93-98.
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