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题名: Bacterial diversity of Taxus rhizosphere: culture-independent and culture-dependent approaches
作者: Hao, Da Cheng2, 3;  Ge, Guang Bo1;  Yang, Ling1
通讯作者: 杨半
关键词: bacterial diversity ;  phylogeny ;  Taxus rhizosphere ;  7-xylosyltaxane-hydrolyzing bacteria
刊名: FEMS MICROBIOLOGY LETTERS
发表日期: 2008-07-01
DOI: 10.1111/j.1574-6968.2008.01201.x
卷: 284, 期:2, 页:204-212
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1806
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: The regional variability of Taxus rhizosphere bacterial community composition and diversity was studied by comparative analysis of three large 16S rRNA gene clone libraries from the Taxus rhizosphere in different regions of China (subtropical and temperate regions). One hundred and forty-six clones were screened for three libraries. Phylogenetic analysis of 16S rRNA gene sequences demonstrated that the abundance of sequences affiliated with Gammaproteobacteria, Betaproteobacteria, and Actinobacteria was higher in the library from the T. x media rhizosphere of the temperate region compared with the subtropical Taxus mairei rhizosphere. On the other hand, Acidobacteria was more abundant in libraries from the subtropical Taxus mairei rhizosphere. Richness estimates and diversity indices of three libraries revealed major differences, indicating a higher richness in the Taxus rhizosphere bacterial communities of the subtropical region and considerable variability in the bacterial community composition within this region. By enrichment culture, a novel Actinobacteria strain DICP16 was isolated from the T. x media rhizosphere of the temperate region and was identified as Leifsonia shinshuensis sp. via 16S rRNA gene and gyrase B sequence analyses. DICP16 was able to remove the xylosyl group from 7-xylosyl-10-deacetylbaccatin III and 7-xylosyl-10-deacetylpaclitaxel, thereby making the xylosyltaxanes available as sources of 10-deacetylbaccatin III and the anticancer drug paclitaxel. Taken together, the present studies provide, for the first time, the knowledge of the biodiversity of microorganisms populating Taxus rhizospheres.
关键词[WOS]: MICROBIAL COMMUNITIES ;  RIBOSOMAL-RNA ;  FOREST SOILS ;  CULTIVATION ;  BIOCONTROL ;  SEEDLINGS ;  DATABASE ;  IMPACT
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000256614300012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/100849
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Jiaotong Univ, Biotechnol Lab, Dalian, Peoples R China
3.Chinese Acad Med Sci, Inst Med Plants, Dalian, Peoples R China

Recommended Citation:
Hao, Da Cheng,Ge, Guang Bo,Yang, Ling. Bacterial diversity of Taxus rhizosphere: culture-independent and culture-dependent approaches[J]. FEMS MICROBIOLOGY LETTERS,2008,284(2):204-212.
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