中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi
作者: Zhao, Xin1, 3;  Kong, Xiangli2;  Hua, Yanyan1;  Feng, Bin2;  Zhao, Zongbao (Kent)1
通讯作者: 赵宗保
关键词: co-fermentation ;  Lipomyces starkeyi ;  microbial oil ;  oleaginous yeast ;  response surface methodology
刊名: EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
发表日期: 2008-05-01
DOI: 10.1002/ejlt.200700224
卷: 110, 期:5, 页:405-412
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Food Science & Technology ;  Nutrition & Dietetics
研究领域[WOS]: Food Science & Technology ;  Nutrition & Dietetics
英文摘要: Co-fermentation of lignocellulose-based carbohydrates is a potential solution to improve the economics of microbial lipid production. In the present paper, experiments were performed to optimize the media composition for lipid production by the oleaginous yeast Lipomyces starkeyi AS 2.1560 through co-fermentation of glucose and xylose (2 : 1 wt/wt). Statistical screening of nine media variables was performed by a Plackett-Burman design. Three factors, namely mixed sugar, yeast extract and FeSO4, were found as significant components influencing cellular lipid accumulation. Further optimization was carried out using a Box-Behnken factorial design to study the effects of these three variables on lipid production. A mathematical model with the R-2 value at 96.66% was developed to show the effect of each medium composition and their interactions on the lipid production. The model estimated that a maximal lipid content of 61.0 wt-% could be obtained when the concentrations of mixed sugar, yeast extract and FeSO4 were at 73.3 g/L (glucose 48.9 g/L, xylose 24.4 g/L), 7.9 g/L and 4.0 mg/L, respectively. The predicted value was in good accordance with the experimental data of 61.5%. Compared with the initial media, the optimized media gave 1.59-fold and 2.03-fold increases for lipid content and lipid productivity, respectively.
关键词[WOS]: RESPONSE-SURFACE METHODOLOGY ;  STAGE CONTINUOUS-CULTURE ;  FED-BATCH CULTURE ;  YARROWIA-LIPOLYTICA ;  CRYPTOCOCCUS CURVATUS ;  APIOTRICHUM-CURVATUM ;  BIODIESEL PRODUCTION ;  DENSITY CULTIVATION ;  MICROORGANISMS ;  ACCUMULATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000256537000005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101145
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Liaoning Normal Univ, Coll Life Sci, Dalian, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Zhao, Xin,Kong, Xiangli,Hua, Yanyan,et al. Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi[J]. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2008,110(5):405-412.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhao, Xin]'s Articles
 [Kong, Xiangli]'s Articles
 [Hua, Yanyan]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhao, Xin]‘s Articles
 [Kong, Xiangli]‘s Articles
 [Hua, Yanyan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace