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题名: RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model
作者: Hu, Chunxiu1, 2;  van Dommelen, Judith1;  van der Heijden, Rob1;  Spijksma, Gerwin1;  Reijmers, Theo H.1;  Wang, Mei3, 4;  Slee, Elizabeth5;  Lu, Xin5;  Xu, Guowang2;  van der Greef, Jan1, 3, 4;  Hankemeier, Thomas1
通讯作者: 许国旺
关键词: Lipidomics ;  Lipid Profiling ;  Metabolite Profiling ;  Metabolomics ;  FTMS ;  p53 Mouse Model
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2008-11-01
DOI: 10.1021/pr800373m
卷: 7, 期:11, 页:4982-4991
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 2;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C(8) column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity (R(2), 0.994-1.000), limit of detection (0.08-1.28 mu g/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TANDEM MASS-SPECTROMETRY ;  BRONCHOALVEOLAR LAVAGE FLUID ;  BIOLOGICAL SAMPLES ;  MEMBRANE PHOSPHOLIPIDS ;  QUANTITATIVE-ANALYSIS ;  CRUDE EXTRACTS ;  IDENTIFICATION ;  MS ;  PHOSPHATIDYLCHOLINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000260792000038
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101153
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Leiden Univ, LACDR, Div Analyt Biosci, NL-2300 RA Leiden, Netherlands
2.Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.SU BioMed, NL-3700 AJ Zeist, Netherlands
4.TNO Qual Life, NL-3700 AJ Zeist, Netherlands
5.Univ Oxford, Ludwig Inst Canc Res, Oxford OX3 7DQ, England

Recommended Citation:
Hu, Chunxiu,van Dommelen, Judith,van der Heijden, Rob,et al. RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model[J]. JOURNAL OF PROTEOME RESEARCH,2008,7(11):4982-4991.
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