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Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups
Liang, Aiye1,2; Liu, Xiaojun1,2; Du, Yuguang1; Wang, Keyi3; Lin, Bingcheng1; Lin BC(林炳承)
关键词Capillary Zone Electrophoresis Granulocyte Colony-stimulating Factor Heparin Interaction Sulfate Group
刊名ELECTROPHORESIS
2008-03-01
DOI10.1002/elps.200700480
29期:6页:1286-1290
收录类别SCI
文章类型Article
部门归属18
项目归属1807,1805
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]FIBROBLAST-GROWTH-FACTOR ; PERFORMANCE FRONTAL ANALYSIS ; HERPES-SIMPLEX-VIRUS ; AFFINITY CAPILLARY-ELECTROPHORESIS ; IN-VIVO ; RECEPTOR ; COMPLEX ; PROTEIN ; IDENTIFICATION ; PROTEOGLYCAN
英文摘要Heparin mediates fundamental biological mechanisms through interaction with proteins. Previously, we have shown that standard heparin binds to granulocyte colony-stimulating factor (G-CSF) with an affinity of 4.8 x 10(5) M-1. To further study the structural features in heparin that are responsible for this interaction, we studied the bindings of G-CSF and N-desulfated and 2,3-O-desulfated heparin by CZE. Results showed that the N-desulfated heparin had a similar affinity for G-CSF ((5.4 +/- 0.9) x 10(5) M-1), but the 2,3-O-desulfated heparin had a 1000-fold lower affinity ((3.4 +/- 1.2) x 10(2) M-1) in comparison to standard heparin. The results showed that 2,3-O-sulfate groups are more important than N-sulfate groups in heparin-G-CSF interaction.
语种英语
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WOS记录号WOS:000254882800010
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101183
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China
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GB/T 7714
Liang, Aiye,Liu, Xiaojun,Du, Yuguang,et al. Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups[J]. ELECTROPHORESIS,2008,29(6):1286-1290.
APA Liang, Aiye,Liu, Xiaojun,Du, Yuguang,Wang, Keyi,Lin, Bingcheng,&林炳承.(2008).Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups.ELECTROPHORESIS,29(6),1286-1290.
MLA Liang, Aiye,et al."Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups".ELECTROPHORESIS 29.6(2008):1286-1290.
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