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题名: Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups
作者: Liang, Aiye1, 2;  Liu, Xiaojun1, 2;  Du, Yuguang1;  Wang, Keyi3;  Lin, Bingcheng1
通讯作者: 林炳承
关键词: capillary zone electrophoresis ;  granulocyte colony-stimulating factor ;  heparin ;  interaction ;  sulfate group
刊名: ELECTROPHORESIS
发表日期: 2008-03-01
DOI: 10.1002/elps.200700480
卷: 29, 期:6, 页:1286-1290
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807,1805
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Heparin mediates fundamental biological mechanisms through interaction with proteins. Previously, we have shown that standard heparin binds to granulocyte colony-stimulating factor (G-CSF) with an affinity of 4.8 x 10(5) M-1. To further study the structural features in heparin that are responsible for this interaction, we studied the bindings of G-CSF and N-desulfated and 2,3-O-desulfated heparin by CZE. Results showed that the N-desulfated heparin had a similar affinity for G-CSF ((5.4 +/- 0.9) x 10(5) M-1), but the 2,3-O-desulfated heparin had a 1000-fold lower affinity ((3.4 +/- 1.2) x 10(2) M-1) in comparison to standard heparin. The results showed that 2,3-O-sulfate groups are more important than N-sulfate groups in heparin-G-CSF interaction.
关键词[WOS]: FIBROBLAST-GROWTH-FACTOR ;  PERFORMANCE FRONTAL ANALYSIS ;  HERPES-SIMPLEX-VIRUS ;  AFFINITY CAPILLARY-ELECTROPHORESIS ;  IN-VIVO ;  RECEPTOR ;  COMPLEX ;  PROTEIN ;  IDENTIFICATION ;  PROTEOGLYCAN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000254882800010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101183
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China

Recommended Citation:
Liang, Aiye,Liu, Xiaojun,Du, Yuguang,et al. Further characterization of the binding of heparin to granulocyte colony-stimulating factor: Importance of sulfate groups[J]. ELECTROPHORESIS,2008,29(6):1286-1290.
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