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题名: Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS
作者: Zhou, Xiaomian;  Shen, Zheng;  Li, Dazhi;  He, Xinya;  Lin, Bingcheng
通讯作者: 林炳承
关键词: microchip non-gel sieving electrophoresis ;  actinomycin D ;  DNA ;  interaction ;  electrospray ionization mass spectrometry
刊名: TALANTA
发表日期: 2007-04-30
DOI: 10.1016/j.talanta.2006.11.019
卷: 72, 期:2, 页:561-567
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 2;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: fwn the present study, the interactions between actinomycin D (ActD) and single stranded DNA (ssDNA) 5'-CGTAACCAACTGCAACGT-3 and a duplex stranded DNA (dsDNA) with this sequence were investigated by microchip-based non-gel sieving electrophoresis and electrospray ionization mass spectrometry (ESI-MS). The ssDNA was designed according to the conserved regions of open reading frame lb (replicase 113) following the Tor 2 SARS genome sequence of 15611-15593. The binding constants of the interactions between ActD and ssDNA/dsDNA were (8.3 +/- 0.32) x 10(6) M-1 (ssDNA) and (2.8 +/- 0.02) x 10(5)M(-1) (dsDNA), respectively, calculated from microchip electrophoresis via Scatchard plot. The binding stoichiometries were 1: 1 (single/1ActD molecule) and 1:2 (duplex/2ActD molecules) calculated from microchip electrophoresis, and the results were further verified by ESI-MS. The results obtained by these two methods indicated that ActD) bound much more tightly to ssDNA used in this work than dsDNA. Furthermore, this is shown that the microchip-based non-gel sieving electrophoresis method is a rapid, highly sensitive and convenient method for the studies of interactions between DNA and small molecule drugs. (c) 2006 Published by Elsevier B.V.
关键词[WOS]: AFFINITY CAPILLARY-ELECTROPHORESIS ;  SINGLE-STRANDED-DNA ;  MASS-SPECTROMETRY ;  MICROFLUIDIC CHIP ;  FLUORESCENCE DETECTION ;  NONCOVALENT COMPLEXES ;  ZONE-ELECTROPHORESIS ;  DRUG-INTERACTION ;  BINDING DRUGS ;  OLIGODEOXYNUCLEOTIDES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000246321200034
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101195
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Guangzhou First Municipal Peoples Hosp, Affiliated Guangzhou Med Coll, Dept Lab Med, Guangzhou 510180, Peoples R China
2.Chinese Acad Sci, Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhou, Xiaomian,Shen, Zheng,Li, Dazhi,et al. Study of interactions between actinomycin D and oligonucleotides by microchip electrophoresis and ESI-MS[J]. TALANTA,2007,72(2):561-567.
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