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题名: Template enhanced activity of lipase accommodated in siliceous mesocellular foams
作者: Zhang, Yanmei2;  Zhao, Lifang1;  Li, Jun2;  Zhang, Huidong2;  Zheng, Liangyu1;  Cao, Shugui1;  Li, Can2
通讯作者: 李灿
关键词: lipase ;  Pseudomonas cepacia ;  siliceous mesocellular foam ;  non-ionic surfactant ;  pluronic P123 ;  template ;  hydrolytic activity
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2008-08-08
DOI: 10.1016/j.bbrc.2008.05.089
卷: 372, 期:4, 页:650-655
收录类别: SCI
文章类型: Article
部门归属: 5
项目归属: 503
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics
英文摘要: Lipases were adsorbed in siliceous mesocellular foams containing different amounts of residual template in the nanopores. It is found that the hydrolytic activities of the adsorbed lipases are increased with increasing the contents of template in the mesopores. The triacetin hydrolytic activity of the lipase adsorbed in the foam containing 46% of template can be 13 times higher than that of the lipase adsorbed in the foam without template in the nanopores, and its specific activity is about three times higher than that of the free lipase, showing the hyperactivation effect on lipase resulting from the interaction between the lipase and the surfactant in the nanopores. The immobilized lipase cross-linked with glutaraldehyde can retain up to 88% of its original activity after six hydrolysis reaction test. This work provides a new strategy to enhance the activity of immobilized lipase in mesoporous materials. (c) 2008 Elsevier Inc. All rights reserved.
关键词[WOS]: OXIDATIVE DEHYDROGENATION ;  CATALYTIC-PROPERTIES ;  IMMOBILIZATION ;  ADSORPTION ;  STABILIZATION ;  BIOCATALYSTS ;  DETERGENTS ;  ACTIVATION ;  RESOLUTION ;  SURFACES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000257419500026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101283
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Yanmei,Zhao, Lifang,Li, Jun,et al. Template enhanced activity of lipase accommodated in siliceous mesocellular foams[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,372(4):650-655.
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