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题名: Spectroscopic studies on the interaction between CdTe nanoparticles and lysozyme
作者: Wu, Yun-Li1;  He, Fei1;  He, Xi-Wen1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
通讯作者: He Xi-Wen
关键词: CdTe nanoparticles ;  Lysozyme ;  Interaction ;  Fluorescence spectroscopy ;  Circular dichroism spectroscopy
刊名: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
发表日期: 2008-12-15
DOI: 10.1016/j.saa.2008.03.018
卷: 71, 期:4, 页:1199-1203
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2;5
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Spectroscopy
研究领域[WOS]: Spectroscopy
英文摘要: Nanoparticles of cadmium telluride (CdTe) coated with thioglycolic acid (TGA) were prepared in the water phase. The interaction between CdTe nanoparticles (NPs) and lysozyme (Lyz) was investigated by fluorescence and circular dichroism (CD) spectroscopy at pH 7.40. It was proved that the fluorescence quenching of Lyz by CdTe NPs was mainly a result of the formation of CdTe-Lyz complex. By the fluorescence quenching results, the Stern-Volmer quenching constant (K(sv)), binding constant (K(a)) and binding sites (n) were calculated. The binding distance (r) between Lyz (the donor) and CdTe NPs (the acceptor) was obtained according to fluorescence resonance energy transfer (FRET). Gradual addition of CdTe NPs to the solution of Lyz led to a marked increase in fluorescence polarization (P) of Lyz, which indicated that CdTe NPs were located in a restricted environment of Lyz. The effect of CdTe NPs on the conformation of Lyz has been analyzed by means of synchronous fluorescence spectra and CD spectra, which provided the evidence that the secondary structure of Lyz has been changed by the interaction of CdTe NPs with Lyz. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: BOVINE SERUM-ALBUMIN ;  FLUORESCENCE SPECTROSCOPY ;  QUANTUM DOTS ;  BINDING ;  NANOCRYSTALS ;  ACID
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000261249100004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101411
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res &Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Wu, Yun-Li,He, Fei,He, Xi-Wen,et al. Spectroscopic studies on the interaction between CdTe nanoparticles and lysozyme[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2008,71(4):1199-1203.
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