DICP OpenIR
Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography
Zheng Chao1; Liu Zhao-Sheng1; Gao Ru-Yu2; Zhang Yu-Kui2,3
KeywordMolecularly Imprinted Polymer Monolith Capillary Electrochromatography Yplg (Tyr-pro-leu-gly) Recognition Mechanism
Source PublicationCHINESE JOURNAL OF CHEMISTRY
2008-10-01
Volume26Issue:10Pages:1857-1862
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordCHIRAL RECOGNITION ; EPITOPE APPROACH ; POLYMERS ; SEPARATION ; TEMPERATURE ; COLUMN ; ENANTIOMERS ; GEL ; RETENTION ; OXYTOCIN
AbstractA tetrapeptide-imprinted capillary monolithic column was synthesized successfully in the presence of YPLG (Tyr-Pro-Leu-Gly) as template by in situ thermo-initiated polymerization. For exploring the recognition mechanism of molecularly imprinted polymer in a capillary electrochromatography (CEC) mode, YPLG and its structural analogue, YPGL (Tyr-Pro-Gly-Leu), differing in amino acid sequence, were used as model compounds. It is hard work to separate the two tetrapeptides by a simple CEC or a capillary electrophoresis mode due to their similar chemical structures and values of pI. However, the results showed that YPLG could be recognized specially by the imprinted monolith. The separation factor (alpha') and the resolution (R(s)) between YPLG and YPGL were 1.73 and 3.72, respectively. The effects of organic modifier concentration, pH value of buffer, salt concentration of buffer and column temperature on the recognition of YPLG and YPGL were investigated. A non-linear Van't Hoff behavior of the template was observed on a YPLG-imprinted column, which suggested the presence of multiple types of retention mechanisms or multiple types of binding sites. The results showed that the molecular recognition on MIP monolith in the CEC mode mainly derived from hydrogen bonding between YPLG and MIP and three-dimensional structure of the imprinting cavities.
Language英语
URL查看原文
WOS IDWOS:000260702500018
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/101413
Collection中国科学院大连化学物理研究所
Corresponding AuthorGao Ru-Yu
Affiliation1.Tianjin Med Univ, Coll Pharm, Tianjin 300070, Peoples R China
2.Nankai Univ, State Key Lab Element Organ Chem, Inst Element Organ Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zheng Chao,Liu Zhao-Sheng,Gao Ru-Yu,et al. Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography[J]. CHINESE JOURNAL OF CHEMISTRY,2008,26(10):1857-1862.
APA Zheng Chao,Liu Zhao-Sheng,Gao Ru-Yu,&Zhang Yu-Kui.(2008).Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography.CHINESE JOURNAL OF CHEMISTRY,26(10),1857-1862.
MLA Zheng Chao,et al."Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography".CHINESE JOURNAL OF CHEMISTRY 26.10(2008):1857-1862.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zheng Chao]'s Articles
[Liu Zhao-Sheng]'s Articles
[Gao Ru-Yu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zheng Chao]'s Articles
[Liu Zhao-Sheng]'s Articles
[Gao Ru-Yu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zheng Chao]'s Articles
[Liu Zhao-Sheng]'s Articles
[Gao Ru-Yu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.