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Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography
Zheng Chao1; Liu Zhao-Sheng1; Gao Ru-Yu2; Zhang Yu-Kui2,3
关键词Molecularly Imprinted Polymer Monolith Capillary Electrochromatography Yplg (Tyr-pro-leu-gly) Recognition Mechanism
刊名CHINESE JOURNAL OF CHEMISTRY
2008-10-01
26期:10页:1857-1862
收录类别SCI
文章类型Article
部门归属18
项目归属1810
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]CHIRAL RECOGNITION ; EPITOPE APPROACH ; POLYMERS ; SEPARATION ; TEMPERATURE ; COLUMN ; ENANTIOMERS ; GEL ; RETENTION ; OXYTOCIN
英文摘要A tetrapeptide-imprinted capillary monolithic column was synthesized successfully in the presence of YPLG (Tyr-Pro-Leu-Gly) as template by in situ thermo-initiated polymerization. For exploring the recognition mechanism of molecularly imprinted polymer in a capillary electrochromatography (CEC) mode, YPLG and its structural analogue, YPGL (Tyr-Pro-Gly-Leu), differing in amino acid sequence, were used as model compounds. It is hard work to separate the two tetrapeptides by a simple CEC or a capillary electrophoresis mode due to their similar chemical structures and values of pI. However, the results showed that YPLG could be recognized specially by the imprinted monolith. The separation factor (alpha') and the resolution (R(s)) between YPLG and YPGL were 1.73 and 3.72, respectively. The effects of organic modifier concentration, pH value of buffer, salt concentration of buffer and column temperature on the recognition of YPLG and YPGL were investigated. A non-linear Van't Hoff behavior of the template was observed on a YPLG-imprinted column, which suggested the presence of multiple types of retention mechanisms or multiple types of binding sites. The results showed that the molecular recognition on MIP monolith in the CEC mode mainly derived from hydrogen bonding between YPLG and MIP and three-dimensional structure of the imprinting cavities.
语种英语
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WOS记录号WOS:000260702500018
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101413
专题中国科学院大连化学物理研究所
通讯作者Gao Ru-Yu
作者单位1.Tianjin Med Univ, Coll Pharm, Tianjin 300070, Peoples R China
2.Nankai Univ, State Key Lab Element Organ Chem, Inst Element Organ Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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Zheng Chao,Liu Zhao-Sheng,Gao Ru-Yu,et al. Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography[J]. CHINESE JOURNAL OF CHEMISTRY,2008,26(10):1857-1862.
APA Zheng Chao,Liu Zhao-Sheng,Gao Ru-Yu,&Zhang Yu-Kui.(2008).Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography.CHINESE JOURNAL OF CHEMISTRY,26(10),1857-1862.
MLA Zheng Chao,et al."Mechanism of Molecular Recognition on Tetrapetide-imprinted Monolith by Capillary Electrochromatography".CHINESE JOURNAL OF CHEMISTRY 26.10(2008):1857-1862.
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