中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin
作者: Wang, Huafang1;  Li, Wenyou1;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 2
通讯作者: He ;  Xiwen
关键词: molecularly imprinted polymer ;  m-Aminophenylboronic acid ;  protein ;  adsorption capacity ;  bovine serum albumin (BSA)
刊名: REACTIVE & FUNCTIONAL POLYMERS
发表日期: 2008-09-01
DOI: 10.1016/j.reactfunctpolym.2008.06.002
卷: 68, 期:9, 页:1291-1296
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Applied ;  Engineering, Chemical ;  Polymer Science
研究领域[WOS]: Chemistry ;  Engineering ;  Polymer Science
英文摘要: Using m-aminophenyl boronic acid (APBA) as a functional monomer, molecularly imprinted polymer (MIP) imprinted with bovine serum albumin (BSA) was fabricated on activated glass spheres under optimized conditions. Key factors in the prepolymerization reaction (between APBA and BSA), such as buffer pH, ionic strength and reaction time, were carefully optimized as previously reported [H.F. Wang, W.Y. Li, X. W. He, et al. Acta Chim. Sinica 65 (2007) 43-48]. The interaction between APBA and BSA during the prepolymerization stage was investigated and optimized, and ideal conditions for protein rebinding experiments were determined as well. Protein rebinding and enriching properties of polymers were studied in single and competitive binding protocols, respectively. The key point of the present paper is that the binding selectivity of polymers may be estimated by the amount of bound-protein recovered from a protein-saturated polymer. Results demonstrated that the selectivity of MIP towards its template protein is superior to that of non-imprinted polymer (NIP). (C) 2008 Elsevier Ltd. All rights reserved.
关键词[WOS]: CHITOSAN BEADS ;  ADSORPTIVE SEPARATION ;  SELECTIVE SEPARATION ;  PROTEIN RECOGNITION ;  IONIC-STRENGTH ;  HEMOGLOBIN ;  ANTIBODIES ;  PARTICLES ;  BINDING ;  SITES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000259655400001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101415
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Nankai Univ, Dept Chem, Minist Educ China, Key Lab Funct Polymer Mat, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Wang, Huafang,Li, Wenyou,He, Xiwen,et al. m-Aminophenylboronic acid as a functional monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin[J]. REACTIVE & FUNCTIONAL POLYMERS,2008,68(9):1291-1296.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang, Huafang]'s Articles
 [Li, Wenyou]'s Articles
 [He, Xiwen]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang, Huafang]‘s Articles
 [Li, Wenyou]‘s Articles
 [He, Xiwen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace