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题名: Preparation and characterization of CdHgTe nanoparticles and their application on the determination of proteins
作者: Mei, Fang1;  He, Xi-Wen1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
通讯作者: He Xi-Wen
关键词: CdHgTe nanoparticles ;  near-infrared ;  fluorescence probe ;  protein ;  determination
刊名: JOURNAL OF FLUORESCENCE
发表日期: 2008-09-01
DOI: 10.1007/s10895-008-0318-1
卷: 18, 期:5, 页:883-890
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2;4
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: CdHgTe nanoparticles ( NPs) with the emission in the near-infrared regions were prepared in aqueous solution, and were characterized by transmission electron microscopy, X-ray diffraction spectrometry, spectrofluorometry and ultraviolet-visible spectrometry. Based on the fluorescence quenching of CdHgTe NPs in the presence of proteins, a novel method for the determination of proteins with CdHgTe NPs as a near-infrared fluorescence probe was developed. Maximum fluorescence quenching was observed with the excitation and emission wavelengths of 500 and 693 nm, respectively. Under the optimal conditions, the calibration graphs were linear in the range of 0.04 x 10(-6) -5.6 x 10(-6) g ml(-1) for lysozyme (Lyz) and 0.06 x 10(-6) -6.1 x 10(-6) g ml(-1) for bovine hemoglobin (BHb), respectively. The limits of detection were 13 ng ml(-1) for Lyz and 27 ng ml(-1) for BHb, respectively. Four synthetic samples were determined and the results were satisfied.
关键词[WOS]: INDUCED FLUORESCENCE DETECTION ;  NEAR-INFRARED LUMINESCENCE ;  QUANTUM DOTS ;  FLUOROMETRIC-DETERMINATION ;  CAPILLARY-ELECTROPHORESIS ;  AQUEOUS-SOLUTION ;  NANOCRYSTALS ;  LYSOZYME ;  ALBUMIN ;  LASER
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000259187600016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101417
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Mei, Fang,He, Xi-Wen,Li, Wen-You,et al. Preparation and characterization of CdHgTe nanoparticles and their application on the determination of proteins[J]. JOURNAL OF FLUORESCENCE,2008,18(5):883-890.
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