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题名: Profiling of Endogenous Serum Phosphorylated Peptides by Titanium (IV) Immobilized Mesoporous Silica Particles Enrichment and MALDI-TOFMS Detection
作者: Hu, Lianghai1;  Zhou, Houjiang1;  Li, Yinghua2;  Sun, Shutao1;  Guo, Lihai5;  Ye, Mingliang1;  Tian, Xiaofeng2;  Gu, Jianren3;  Yang, Shengli1, 4;  Zou, Hanfa1
通讯作者: 邹汉法
刊名: ANALYTICAL CHEMISTRY
发表日期: 2009
DOI: 10.1021/ac801974f
卷: 81, 期:1, 页:94-104
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Phosphorylation is one of the most important post-translational modifications of proteins, which modulates a wide range of biological functions and activities of proteins. The phosphorylation of proteins is also associated with the pathway of cancer cells. We have previously enriched the low molecular weight proteome from human plasma based on the combination of size exclusion and adsorption mechanism by using highly ordered mesoporous silica particles. Herein, highly ordered mesoporous silica particles were modified with titanium phosphonate to selectively capture the phosphopeptides from complex peptide and protein mixtures. The limit of detection for phosphopeptides from beta-casein and standard phosphopeptide spiked in human serum was as low as 1.25 fmol based on MALDI-TOFMS detection. The modified mesoporous silica particles were further used to enrich phosphopeptides from serum of hepatocellular carcinoma patients and healthy individuals and then analyzed with MALDI-TOFMS. The combination of isobaric tagging for relative and absolute quantitation labeling with MALDI-TOFMS/MS was further applied to validate the serum phosphopeptide profiling result of MALDI-TOFMS. The profiling of the serum phosphopeptides between the cancer patients and healthy persons was distinguishingly different, which indicated the potential ability of this technique for cancer diagnosis and biomarker discovery. The approach developed here would be applicable to other biological samples and a wide variety of diseases.
关键词[WOS]: IONIZATION MASS-SPECTROMETRY ;  ION AFFINITY-CHROMATOGRAPHY ;  PHOSPHOPROTEOME ANALYSIS ;  MS ANALYSIS ;  OVARIAN-CANCER ;  PHOSPHOPEPTIDE ENRICHMENT ;  CONCENTRATING PROBES ;  SELECTIVE ENRICHMENT ;  RAPID ENRICHMENT ;  NANOPARTICLES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000262113400018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101445
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China
3.Shanghai Jiao Tong Univ, Shanghai Canc Inst, State Key Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China
4.Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai 200032, Peoples R China
5.Appl Biosyst Inc, Asia Pacific Applicat Support Ctr, Shanghai 200040, Peoples R China

Recommended Citation:
Hu, Lianghai,Zhou, Houjiang,Li, Yinghua,et al. Profiling of Endogenous Serum Phosphorylated Peptides by Titanium (IV) Immobilized Mesoporous Silica Particles Enrichment and MALDI-TOFMS Detection[J]. ANALYTICAL CHEMISTRY,2009,81(1):94-104.
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