DICP OpenIR
Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis
Tian, Ruijun2; Ren, Lianbing1; Ma, Huaijun3; Li, Xin2; Hu, Lianghai2; Ye, Mingliang2; Wu, Ren'an2; Tian, Zhijian3; Liu, Zhen1; Zou, Hanfa2; Zou HF(邹汉法)
KeywordPeptidomics Ordered Porous Nanoparticles Strong Ion Exchange Reversed Phase Two-dimensional Separation Mouse Liver
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2009-02-20
ISSN0021-9673
DOI10.1016/j.chroma.2008.10.002
Volume1216Issue:8Pages:1270-1278
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMASS-SPECTROMETRY ; BIOMARKER DISCOVERY ; MESOPOROUS SILICA ; HUMAN PLASMA ; FUNCTIONALIZED MCM-41 ; MEMBRANE-PROTEINS ; PROTEOME ; SERUM ; IDENTIFICATION ; CHROMATOGRAPHY
AbstractWe report the development of a combined strategy for high capacity, comprehensive enrichment of endogenous peptide from complex biological samples at natural pH condition. MCM-41 nanoparticles with highly ordered nanoscale pores (i.e. 4.8 nm) and high-surface area (i.e. 751 m(2)/g) were synthesized and modified with strong cation-exchange (SCX-MCM-41) and strong anion-exchange (SAX-MCM-41) groups. The modified nanoparticles demonstrated good size-exclusion effect for the adsorption of standard protein lysozyme with molecular weight (MW) of ca. 15 kDa; and the peptides with MW lower than this value can be well adsorbed. Step elution of the enriched peptides with five salt concentrations presented that both modified nanoparticles have high capacity and complementarity for peptides enrichment, and the SAX-MCM-41 nanoparticles has obviously high selectivity for acidic peptides with pI (isoelectric point) lower than 4. Large-scale enrichment of endogenous peptides in 2 mg mouse liver extract was achieved by further combination of SCX-MCM-41 and SAX-MCM-41 with unmodified MCM-41 nanoparticles. On-line 2D nano-LC/MS/MS was applied to analyze the enriched samples, and 2721 unique peptides were identified in total. Two-dimensional analysis of MW versus pI distribution combined with abundance of the identified peptides demonstrated that the three types of nanoparticles have comprehensive complementarity for peptidome enrichment. (c) 2008 Published by Elsevier B.V.
Language英语
URL查看原文
WOS IDWOS:000263740300008
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/101509
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法)
Affiliation1.Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116013, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Appl Catalysis, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Tian, Ruijun,Ren, Lianbing,Ma, Huaijun,et al. Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(8):1270-1278.
APA Tian, Ruijun.,Ren, Lianbing.,Ma, Huaijun.,Li, Xin.,Hu, Lianghai.,...&邹汉法.(2009).Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis.JOURNAL OF CHROMATOGRAPHY A,1216(8),1270-1278.
MLA Tian, Ruijun,et al."Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis".JOURNAL OF CHROMATOGRAPHY A 1216.8(2009):1270-1278.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tian, Ruijun]'s Articles
[Ren, Lianbing]'s Articles
[Ma, Huaijun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tian, Ruijun]'s Articles
[Ren, Lianbing]'s Articles
[Ma, Huaijun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tian, Ruijun]'s Articles
[Ren, Lianbing]'s Articles
[Ma, Huaijun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.