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题名: Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis
作者: Tian, Ruijun2;  Ren, Lianbing1;  Ma, Huaijun3;  Li, Xin2;  Hu, Lianghai2;  Ye, Mingliang2;  Wu, Ren'an2;  Tian, Zhijian3;  Liu, Zhen1;  Zou, Hanfa2
通讯作者: 邹汉法
关键词: Peptidomics ;  Ordered porous nanoparticles ;  Strong ion exchange ;  Reversed phase ;  Two-dimensional separation ;  Mouse liver
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-02-20
DOI: 10.1016/j.chroma.2008.10.002
卷: 1216, 期:8, 页:1270-1278
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: We report the development of a combined strategy for high capacity, comprehensive enrichment of endogenous peptide from complex biological samples at natural pH condition. MCM-41 nanoparticles with highly ordered nanoscale pores (i.e. 4.8 nm) and high-surface area (i.e. 751 m(2)/g) were synthesized and modified with strong cation-exchange (SCX-MCM-41) and strong anion-exchange (SAX-MCM-41) groups. The modified nanoparticles demonstrated good size-exclusion effect for the adsorption of standard protein lysozyme with molecular weight (MW) of ca. 15 kDa; and the peptides with MW lower than this value can be well adsorbed. Step elution of the enriched peptides with five salt concentrations presented that both modified nanoparticles have high capacity and complementarity for peptides enrichment, and the SAX-MCM-41 nanoparticles has obviously high selectivity for acidic peptides with pI (isoelectric point) lower than 4. Large-scale enrichment of endogenous peptides in 2 mg mouse liver extract was achieved by further combination of SCX-MCM-41 and SAX-MCM-41 with unmodified MCM-41 nanoparticles. On-line 2D nano-LC/MS/MS was applied to analyze the enriched samples, and 2721 unique peptides were identified in total. Two-dimensional analysis of MW versus pI distribution combined with abundance of the identified peptides demonstrated that the three types of nanoparticles have comprehensive complementarity for peptidome enrichment. (c) 2008 Published by Elsevier B.V.
关键词[WOS]: MASS-SPECTROMETRY ;  BIOMARKER DISCOVERY ;  MESOPOROUS SILICA ;  HUMAN PLASMA ;  FUNCTIONALIZED MCM-41 ;  MEMBRANE-PROTEINS ;  PROTEOME ;  SERUM ;  IDENTIFICATION ;  CHROMATOGRAPHY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000263740300008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101509
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116013, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Appl Catalysis, Dalian 116023, Peoples R China

Recommended Citation:
Tian, Ruijun,Ren, Lianbing,Ma, Huaijun,et al. Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(8):1270-1278.
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