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Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis
Tian, Ruijun2; Ren, Lianbing1; Ma, Huaijun3; Li, Xin2; Hu, Lianghai2; Ye, Mingliang2; Wu, Ren'an2; Tian, Zhijian3; Liu, Zhen1; Zou, Hanfa2; Zou HF(邹汉法)
关键词Peptidomics Ordered Porous Nanoparticles Strong Ion Exchange Reversed Phase Two-dimensional Separation Mouse Liver
刊名JOURNAL OF CHROMATOGRAPHY A
2009-02-20
ISSN0021-9673
DOI10.1016/j.chroma.2008.10.002
1216期:8页:1270-1278
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MASS-SPECTROMETRY ; BIOMARKER DISCOVERY ; MESOPOROUS SILICA ; HUMAN PLASMA ; FUNCTIONALIZED MCM-41 ; MEMBRANE-PROTEINS ; PROTEOME ; SERUM ; IDENTIFICATION ; CHROMATOGRAPHY
英文摘要We report the development of a combined strategy for high capacity, comprehensive enrichment of endogenous peptide from complex biological samples at natural pH condition. MCM-41 nanoparticles with highly ordered nanoscale pores (i.e. 4.8 nm) and high-surface area (i.e. 751 m(2)/g) were synthesized and modified with strong cation-exchange (SCX-MCM-41) and strong anion-exchange (SAX-MCM-41) groups. The modified nanoparticles demonstrated good size-exclusion effect for the adsorption of standard protein lysozyme with molecular weight (MW) of ca. 15 kDa; and the peptides with MW lower than this value can be well adsorbed. Step elution of the enriched peptides with five salt concentrations presented that both modified nanoparticles have high capacity and complementarity for peptides enrichment, and the SAX-MCM-41 nanoparticles has obviously high selectivity for acidic peptides with pI (isoelectric point) lower than 4. Large-scale enrichment of endogenous peptides in 2 mg mouse liver extract was achieved by further combination of SCX-MCM-41 and SAX-MCM-41 with unmodified MCM-41 nanoparticles. On-line 2D nano-LC/MS/MS was applied to analyze the enriched samples, and 2721 unique peptides were identified in total. Two-dimensional analysis of MW versus pI distribution combined with abundance of the identified peptides demonstrated that the three types of nanoparticles have comprehensive complementarity for peptidome enrichment. (c) 2008 Published by Elsevier B.V.
语种英语
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WOS记录号WOS:000263740300008
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被引频次:43[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101509
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
作者单位1.Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116013, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Appl Catalysis, Dalian 116023, Peoples R China
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GB/T 7714
Tian, Ruijun,Ren, Lianbing,Ma, Huaijun,et al. Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(8):1270-1278.
APA Tian, Ruijun.,Ren, Lianbing.,Ma, Huaijun.,Li, Xin.,Hu, Lianghai.,...&邹汉法.(2009).Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis.JOURNAL OF CHROMATOGRAPHY A,1216(8),1270-1278.
MLA Tian, Ruijun,et al."Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis".JOURNAL OF CHROMATOGRAPHY A 1216.8(2009):1270-1278.
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