中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species
作者: Hao, Da Cheng;  Yang, Ling1;  Huang, Beili
通讯作者: 杨凌
关键词: Adaptive evolution ;  10-Deacetylbaccatin III-10 beta-O-acetyltransferase ;  Paclitaxel ;  Positive selection ;  Taxadiene synthase ;  Taxus
刊名: GENETICA
发表日期: 2009-03-01
DOI: 10.1007/s10709-008-9257-7
卷: 135, 期:2, 页:123-135
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1806
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
英文摘要: Evolutionary patterns of sequence divergence were analyzed in genes from the conifer genus Taxus (yew), encoding paclitaxel biosynthetic enzymes taxadiene synthase (TS) and 10-deacetylbaccatin III-10 beta-O-acetyltransferase (DBAT). N-terminal fragments of TS, full-length DBAT and internal transcribed spacer (ITS) were amplified from 15 closely related Taxus species and sequenced. Premature stop codons were not found in TS and DBAT sequences. Codon usage bias was not found, suggesting that synonymous mutations are selectively neutral. TS and DBAT gene trees are not consistent with the ITS tree, where species formed monophyletic clades. In fact, for both genes, alleles were sometimes shared across species and parallel amino acid substitutions were identified. While both TS and DBAT are, overall, under purifying selection, we identified a number of amino acids of TS under positive selection based on inference using maximum likelihood models. Positively selected amino acids in the N-terminal region of TS suggest that this region might be more important for enzyme function than previously thought. Moreover, we identify lineages with significantly elevated rates of amino acid substitution using a genetic algorithm. These findings demonstrate that the pattern of adaptive paclitaxel biosynthetic enzyme evolution can be documented between closely related Taxus species, where species-specific taxane metabolism has evolved recently.
关键词[WOS]: AMINO-ACID SITES ;  TAXOL BIOSYNTHESIS ;  TAXADIENE SYNTHASE ;  SELECTION PRESSURE ;  ESCHERICHIA-COLI ;  COMMITTED STEP ;  NUCLEOTIDE ;  MODELS ;  SUBSTITUTIONS ;  CYCLIZATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000263058600001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101531
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian, Peoples R China

Recommended Citation:
Hao, Da Cheng,Yang, Ling,Huang, Beili. Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species[J]. GENETICA,2009,135(2):123-135.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Hao, Da Cheng]'s Articles
 [Yang, Ling]'s Articles
 [Huang, Beili]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Hao, Da Cheng]‘s Articles
 [Yang, Ling]‘s Articles
 [Huang, Beili]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace