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题名: Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression
作者: Zhao, Peng1;  Zhu, Guijie1;  Zhang, Weibing1;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Binding constant ;  Human serum albumin ;  Dexamethasone ;  Capillary electrophoresis-frontal analysis
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2009
DOI: 10.1007/s00216-008-2373-5
卷: 393, 期:1, 页:257-261
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Studies into the interactions between drugs and human serum albumin (HSA) are extremely important for drug discovery, since HSA behaves as a carrier for external drugs and internal biological molecules. In this paper, to evaluate the pharmacokinetic and pharmacodynamic properties of dexamethasone (DXM), the interaction between DXM and HSA was studied by capillary electrophoresis frontal analysis (CE-FA). According to the Klotz equation, four binding sites between DXM and HSA were obtained, and the average binding constant was 1.05 x 10(3) M(-1). Furthermore, according to multiple equilibrium theory, based on the assumption that there are two types of binding site, the binding constant at one site was calculated to be 3.539 x 10(3) M(-1), and the average of the other three was 1.234 x 10(3) M(-1). In addition, to obtain the detailed binding information at each binding site, new equations were deduced by multivariate regression. The four binding constants of DXM and HSA were calculated to be 5.558 x 10(1) M(-1), 2.158 x 10(4) M(-1), 7.312 x 10(3) M(-1) and 2.043 x 10(3) M(-1), respectively, which is helpful for detailed studies into the interactions between drugs and proteins with multiple binding sites.
关键词[WOS]: CONTACTLESS CONDUCTIVITY DETECTION ;  PROTEIN INTERACTIONS ;  NONCOVALENT INTERACTIONS ;  DRUG ;  MICRODIALYSIS ;  SITES ;  UV
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000261831000029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101555
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Zhao, Peng,Zhu, Guijie,Zhang, Weibing,et al. Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2009,393(1):257-261.
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