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Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression
Zhao, Peng; Zhu, Guijie; Zhang, Weibing; Zhang, Lihua; Liang, Zhen; Zhang, Yukui; Zhang LH(张丽华)
KeywordBinding Constant Human Serum Albumin Dexamethasone Capillary Electrophoresis-frontal Analysis
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2009
DOI10.1007/s00216-008-2373-5
Volume393Issue:1Pages:257-261
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCONTACTLESS CONDUCTIVITY DETECTION ; PROTEIN INTERACTIONS ; NONCOVALENT INTERACTIONS ; DRUG ; MICRODIALYSIS ; SITES ; UV
AbstractStudies into the interactions between drugs and human serum albumin (HSA) are extremely important for drug discovery, since HSA behaves as a carrier for external drugs and internal biological molecules. In this paper, to evaluate the pharmacokinetic and pharmacodynamic properties of dexamethasone (DXM), the interaction between DXM and HSA was studied by capillary electrophoresis frontal analysis (CE-FA). According to the Klotz equation, four binding sites between DXM and HSA were obtained, and the average binding constant was 1.05 x 10(3) M(-1). Furthermore, according to multiple equilibrium theory, based on the assumption that there are two types of binding site, the binding constant at one site was calculated to be 3.539 x 10(3) M(-1), and the average of the other three was 1.234 x 10(3) M(-1). In addition, to obtain the detailed binding information at each binding site, new equations were deduced by multivariate regression. The four binding constants of DXM and HSA were calculated to be 5.558 x 10(1) M(-1), 2.158 x 10(4) M(-1), 7.312 x 10(3) M(-1) and 2.043 x 10(3) M(-1), respectively, which is helpful for detailed studies into the interactions between drugs and proteins with multiple binding sites.
Language英语
URL查看原文
WOS IDWOS:000261831000029
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/101555
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Peng,Zhu, Guijie,Zhang, Weibing,et al. Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2009,393(1):257-261.
APA Zhao, Peng.,Zhu, Guijie.,Zhang, Weibing.,Zhang, Lihua.,Liang, Zhen.,...&张丽华.(2009).Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,393(1),257-261.
MLA Zhao, Peng,et al."Study of multiple binding constants of dexamethasone with human serum albumin by capillary electrophoresis-frontal analysis and multivariate regression".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 393.1(2009):257-261.
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