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题名: Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells
作者: Liu, Hong-Tao1, 2, 3;  Li, Wen-Ming1;  Xu, Gang1;  Li, Xiu-Ying1;  Bai, Xue-Fang2;  Wei, Peng2;  Yu, Chao1;  Du, Yu-Guang2
通讯作者: 杜昱光
关键词: Chitosan oligosaccharides ;  Human umbilical vein endothelial cells ;  Hydrogen peroxide ;  Apoptosis
刊名: PHARMACOLOGICAL RESEARCH
发表日期: 2009-03-01
DOI: 10.1016/j.phrs.2008.12.001
卷: 59, 期:3, 页:167-175
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
英文摘要: Chitosan oligosaccharides (COS) have been reported to have anticancer activity, immuno-enhancing effect and antimicrobial activity. However, other biological activities are unknown. Herein, we investigated the protective effects of COS against hydrogen peroxide (H2O2)-induced oxidative damage on human umbilical vein endothelial cells (HUVEC, ECV304 cells). After 24 h pre-incubation with COS (25-200 mu g/ml), the viability loss in ECV304 cells induced by H2O2(300 mu M) for 12 h was markedly restored in a concentration-dependent manner as measured by MTT assay. This effect was accompanied by a marked decrease in intracellular reactive oxygen species (ROS) by measuring intensity of DCFH fluorescence. COS also exerted preventive effects on suppressing the production of lipid pet-oxidation such as malondialdehyde (MDA), restoring activities of endogenous antioxidants including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), along with the capacity of increasing levels of nitric oxide (NO) and nitric oxide synthase (NOS), as were determined by commercial regent kits. In addition, pre-incubation of COS with ECV304 cells for 24 h resulted in the reduction of apoptosis and the induction of cell cycle arrest in G(1)/S + M phase as assayed quantitatively by Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit using flow cytometry. Taken together, Our findings suggest that COS can effectively protect HUVECs against oxidative stress by H2O2, which might be of importance in the treatment of cardiovascular diseases. (C) 2008 Elsevier Ltd. All rights reserved.
关键词[WOS]: NITRIC-OXIDE SYNTHASE ;  ACTIVATED PROTEIN-KINASE ;  KAPPA-B ACTIVATION ;  IN-VITRO ;  OXIDATIVE STRESS ;  PC12 CELLS ;  H2O2-INDUCED APOPTOSIS ;  ANTIOXIDANT ACTIVITIES ;  SHEAR-STRESS ;  CHITOOLIGOSACCHARIDES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000264615100004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101557
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chongqing Med Univ, Inst Basic Med Sci, Chongqing 400016, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 110864, Peoples R China

Recommended Citation:
Liu, Hong-Tao,Li, Wen-Ming,Xu, Gang,et al. Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells[J]. PHARMACOLOGICAL RESEARCH,2009,59(3):167-175.
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