DICP OpenIR
Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells
Liu, Hong-Tao1,2,3; Li, Wen-Ming1; Xu, Gang1; Li, Xiu-Ying1; Bai, Xue-Fang2; Wei, Peng2; Yu, Chao1; Du, Yu-Guang2; Du YG(杜昱光)
关键词Chitosan Oligosaccharides Human Umbilical Vein Endothelial Cells Hydrogen Peroxide Apoptosis
刊名PHARMACOLOGICAL RESEARCH
2009-03-01
ISSNISSN:1043-6618
DOI10.1016/j.phrs.2008.12.001
59期:3页:167-175
收录类别SCI
文章类型Article
部门归属18
项目归属1805
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]NITRIC-OXIDE SYNTHASE ; ACTIVATED PROTEIN-KINASE ; KAPPA-B ACTIVATION ; IN-VITRO ; OXIDATIVE STRESS ; PC12 CELLS ; H2O2-INDUCED APOPTOSIS ; ANTIOXIDANT ACTIVITIES ; SHEAR-STRESS ; CHITOOLIGOSACCHARIDES
英文摘要Chitosan oligosaccharides (COS) have been reported to have anticancer activity, immuno-enhancing effect and antimicrobial activity. However, other biological activities are unknown. Herein, we investigated the protective effects of COS against hydrogen peroxide (H2O2)-induced oxidative damage on human umbilical vein endothelial cells (HUVEC, ECV304 cells). After 24 h pre-incubation with COS (25-200 mu g/ml), the viability loss in ECV304 cells induced by H2O2(300 mu M) for 12 h was markedly restored in a concentration-dependent manner as measured by MTT assay. This effect was accompanied by a marked decrease in intracellular reactive oxygen species (ROS) by measuring intensity of DCFH fluorescence. COS also exerted preventive effects on suppressing the production of lipid pet-oxidation such as malondialdehyde (MDA), restoring activities of endogenous antioxidants including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), along with the capacity of increasing levels of nitric oxide (NO) and nitric oxide synthase (NOS), as were determined by commercial regent kits. In addition, pre-incubation of COS with ECV304 cells for 24 h resulted in the reduction of apoptosis and the induction of cell cycle arrest in G(1)/S + M phase as assayed quantitatively by Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit using flow cytometry. Taken together, Our findings suggest that COS can effectively protect HUVECs against oxidative stress by H2O2, which might be of importance in the treatment of cardiovascular diseases. (C) 2008 Elsevier Ltd. All rights reserved.
语种英语
原文出处查看原文
WOS记录号WOS:000264615100004
引用统计
被引频次:100[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101557
专题中国科学院大连化学物理研究所
通讯作者Du YG(杜昱光)
作者单位1.Chongqing Med Univ, Inst Basic Med Sci, Chongqing 400016, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 110864, Peoples R China
推荐引用方式
GB/T 7714
Liu, Hong-Tao,Li, Wen-Ming,Xu, Gang,et al. Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells[J]. PHARMACOLOGICAL RESEARCH,2009,59(3):167-175.
APA Liu, Hong-Tao.,Li, Wen-Ming.,Xu, Gang.,Li, Xiu-Ying.,Bai, Xue-Fang.,...&杜昱光.(2009).Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells.PHARMACOLOGICAL RESEARCH,59(3),167-175.
MLA Liu, Hong-Tao,et al."Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells".PHARMACOLOGICAL RESEARCH 59.3(2009):167-175.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Hong-Tao]的文章
[Li, Wen-Ming]的文章
[Xu, Gang]的文章
百度学术
百度学术中相似的文章
[Liu, Hong-Tao]的文章
[Li, Wen-Ming]的文章
[Xu, Gang]的文章
必应学术
必应学术中相似的文章
[Liu, Hong-Tao]的文章
[Li, Wen-Ming]的文章
[Xu, Gang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。