DICP OpenIR
Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells
Liu, Hong-Tao1,2,3; Li, Wen-Ming1; Xu, Gang1; Li, Xiu-Ying1; Bai, Xue-Fang2; Wei, Peng2; Yu, Chao1; Du, Yu-Guang2; Du YG(杜昱光)
KeywordChitosan Oligosaccharides Human Umbilical Vein Endothelial Cells Hydrogen Peroxide Apoptosis
Source PublicationPHARMACOLOGICAL RESEARCH
2009-03-01
ISSNISSN:1043-6618
DOI10.1016/j.phrs.2008.12.001
Volume59Issue:3Pages:167-175
Indexed BySCI
SubtypeArticle
Department18
Funding Project1805
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectPharmacology & Pharmacy
WOS Research AreaPharmacology & Pharmacy
WOS KeywordNITRIC-OXIDE SYNTHASE ; ACTIVATED PROTEIN-KINASE ; KAPPA-B ACTIVATION ; IN-VITRO ; OXIDATIVE STRESS ; PC12 CELLS ; H2O2-INDUCED APOPTOSIS ; ANTIOXIDANT ACTIVITIES ; SHEAR-STRESS ; CHITOOLIGOSACCHARIDES
AbstractChitosan oligosaccharides (COS) have been reported to have anticancer activity, immuno-enhancing effect and antimicrobial activity. However, other biological activities are unknown. Herein, we investigated the protective effects of COS against hydrogen peroxide (H2O2)-induced oxidative damage on human umbilical vein endothelial cells (HUVEC, ECV304 cells). After 24 h pre-incubation with COS (25-200 mu g/ml), the viability loss in ECV304 cells induced by H2O2(300 mu M) for 12 h was markedly restored in a concentration-dependent manner as measured by MTT assay. This effect was accompanied by a marked decrease in intracellular reactive oxygen species (ROS) by measuring intensity of DCFH fluorescence. COS also exerted preventive effects on suppressing the production of lipid pet-oxidation such as malondialdehyde (MDA), restoring activities of endogenous antioxidants including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), along with the capacity of increasing levels of nitric oxide (NO) and nitric oxide synthase (NOS), as were determined by commercial regent kits. In addition, pre-incubation of COS with ECV304 cells for 24 h resulted in the reduction of apoptosis and the induction of cell cycle arrest in G(1)/S + M phase as assayed quantitatively by Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit using flow cytometry. Taken together, Our findings suggest that COS can effectively protect HUVECs against oxidative stress by H2O2, which might be of importance in the treatment of cardiovascular diseases. (C) 2008 Elsevier Ltd. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000264615100004
Citation statistics
Cited Times:105[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/101557
Collection中国科学院大连化学物理研究所
Corresponding AuthorDu YG(杜昱光)
Affiliation1.Chongqing Med Univ, Inst Basic Med Sci, Chongqing 400016, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 110864, Peoples R China
Recommended Citation
GB/T 7714
Liu, Hong-Tao,Li, Wen-Ming,Xu, Gang,et al. Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells[J]. PHARMACOLOGICAL RESEARCH,2009,59(3):167-175.
APA Liu, Hong-Tao.,Li, Wen-Ming.,Xu, Gang.,Li, Xiu-Ying.,Bai, Xue-Fang.,...&杜昱光.(2009).Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells.PHARMACOLOGICAL RESEARCH,59(3),167-175.
MLA Liu, Hong-Tao,et al."Chitosan oligosaccharides attenuate hydrogen peroxide-induced stress injury in human umbilical vein endothelial cells".PHARMACOLOGICAL RESEARCH 59.3(2009):167-175.
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