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题名: Preparation of monodisperse immobilized Ti4+ affinity chromatography microspheres for specific enrichment of phosphopeptides
作者: Yu, Zhiyuan1;  Han, Guanghui1;  Sun, Shutao1;  Jiang, Xinning1;  Chen, Rui1;  Wang, Fangjun1;  Wu, Ren'an1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: Monodisperse ;  Microspheres ;  Phosphopeptides enrichment ;  Phosphoproteome
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2009-03-16
DOI: 10.1016/j.aca.2009.01.033
卷: 636, 期:1, 页:34-41
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: This study presented an approach to prepare monodisperse immobilized Ti4+ affinity chromatography (Ti4+-IMAC) microspheres for specific enrichment of phosphopeptides in phosphoproteome analysis. Monodisperse polystyrene seed microspheres with a diameter of ca. 4.8 mu m were first prepared by a dispersion polymerization method. Monodisperse microspheres with a diameter of ca. 13 mu m were prepared using the seed microspheres by a single-step swelling and polymerization method. Ti4+ ion was immobilized after chemical modification of the microspheres with phosphonate groups. The specificity of the Ti4+-IMAC microspheres to phosphopeptides was demonstrated by selective enrichment of phosphopeptides from Mixture of tryptic digests of alpha-casein and bovine serum albumin (BSA) at molar ratio of 1 to 500 by MALDI-TOF MS analysis. The sensitivity of detection for phosphopeptides determined by MALDI-TOF MS was as low as 5 fmol for standard tryptic digest of beta-casein. The Ti4+-IMAC microspheres were compared with commercial Fe3+-IMAC adsorbent and homemade Zr4+-IMAC microspheres for enrichment of phosphopeptides. The phosphopeptides and non-phosphopeptides identified by Fe3+-IMAC, Zr4+-IMAC and Ti4+-IMAC methods were 26,114,127 and 181,11,11 respectively for the same tryptic digest samples. The results indicated that the Ti4+-IMAC had the best performance for enrichment of phosphopepticles. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  HIGHLY SELECTIVE ENRICHMENT ;  CORE-SHELL MICROSPHERES ;  LINEAR ION-TRAP ;  TOF MS ANALYSIS ;  PHOSPHOPROTEOME ANALYSIS ;  PHOSPHORYLATED PEPTIDES ;  POLY(GLYCIDYLMETHACRYLATE-CO-ETHYLENEDIMETHACRYLATE) BEADS ;  LIQUID-CHROMATOGRAPHY ;  POLYMER PARTICLES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000264212100006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101567
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Yu, Zhiyuan,Han, Guanghui,Sun, Shutao,et al. Preparation of monodisperse immobilized Ti4+ affinity chromatography microspheres for specific enrichment of phosphopeptides[J]. ANALYTICA CHIMICA ACTA,2009,636(1):34-41.
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