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Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposome chromatography column and reversed-phase column for separation of complex traditional Chinese medicine Longdan Xiegan Decoction
Wang, Yun1,2,3; Kong, Liang4; Lei, Xiaoyuan1; Hu, Lianghai1; Zou, Hanfa1; Welbeck, Ed5; Bligh, S. W. Annie5; Wang, Zhengtao2,3; Zou HF(邹汉法); Zhengtao Wang
关键词Comprehensive Two-dimensional Chromatography Ilc Column Biological Finger Printing Analysis Longdan Xiegan Decoction Traditional Chinese Medicine
刊名JOURNAL OF CHROMATOGRAPHY A
2009-03-13
ISSN0021-9673
DOI10.1016/j.chroma.2008.05.074
1216期:11页:2185-2191
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名2;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; PROTEIN ; IDENTIFICATION ; MEMBRANE ; HPLC ; PRESCRIPTION ; DIGESTION
英文摘要A comprehensive two-dimensional HPLC system with an immobilized liposome chromatography (ILC) column in conjunction with all RP column (in tandem) was developed for the screening and analysis of the membrane-permeable Compounds in a traditional Chinese medicine prescription Longdan Xiegan Decoction (LXD). More than 50 components in LXD were resolved using the developed separation system. Eight flavonoids and two iridoids were identified interacting with the ILC column: a system that mimics biomembranes. The results show that the developed comprehensive two-dimensional chromatography system can be used for identifying membrane permeable natural products in complex matrixes such as extracts of traditional Chinese medicine prescriptions. (C) 2008 Published by Elsevier B.V.
语种英语
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WOS记录号WOS:000264226500028
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被引频次:57[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101569
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zhengtao Wang
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Shanghai Univ Tradit Chinese Med, Minist Educat, Key Lab Standuidizat Chinese Med, Shanghai 201210, Peoples R China
3.China Pharmaceut Univ, Dept Pharmacognosy, Nanjing 210038, Peoples R China
4.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China
5.London Metropolitan Univ, Inst Hlth Res & Policy, London N7 8D8, England
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Wang, Yun,Kong, Liang,Lei, Xiaoyuan,et al. Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposome chromatography column and reversed-phase column for separation of complex traditional Chinese medicine Longdan Xiegan Decoction[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2185-2191.
APA Wang, Yun.,Kong, Liang.,Lei, Xiaoyuan.,Hu, Lianghai.,Zou, Hanfa.,...&Zhengtao Wang.(2009).Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposome chromatography column and reversed-phase column for separation of complex traditional Chinese medicine Longdan Xiegan Decoction.JOURNAL OF CHROMATOGRAPHY A,1216(11),2185-2191.
MLA Wang, Yun,et al."Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposome chromatography column and reversed-phase column for separation of complex traditional Chinese medicine Longdan Xiegan Decoction".JOURNAL OF CHROMATOGRAPHY A 1216.11(2009):2185-2191.
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