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Micropumps actuated negative pressure injection for microchip electrophoresis
Li, Bowei1,2; Jiang, Lei1; Wang, Qi3; Oin, Jianhua1; Lin, Bingcheng1; Lin BC(林炳承)
关键词Integrated Micropump Microchip Electrophoresis Negative Pressure Pinched Injection
刊名ELECTROPHORESIS
2008-12-01
DOI10.1002/elps.200800336
29期:24页:4906-4913
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TOTAL ANALYSIS SYSTEMS ; MICROFLUIDIC CHIP ; CAPILLARY ELECTROPHORESIS ; HYDROSTATIC-PRESSURE ; SAMPLE INJECTION ; DEVICE ; SEPARATION ; INTEGRATION ; TECHNOLOGY ; GRAVITY
英文摘要A simple negative pressure pinched sample injection method was presented. This method combined diaphragm micropumps and a single voltage supply to generate controllable well-defined sample plug, and led to effective electrophoresis separation. The pinched plug was found to be favorable for obtaining representative and reproducible results that the RSD of the migration time and peak height of sodium fluorescein were 0.5 and 2.1%, respectively (n = 25). The established method had been applied in separation of amino acid samples. This method has the advantages of well-defined plug, free sample bias effect, high reproducibility and convenience of controlling the negative pressure by the integrated pumps on the microchip. In addition, the single high voltage supply and the world-to-chip interface simplified the instrumentation, which is of benefit to the minimization and automation. These advantages demonstrate the potential of this method for a wide range of applications.
语种英语
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WOS记录号WOS:000262565700011
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101589
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dept Resp Dis, Dalian, Peoples R China
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GB/T 7714
Li, Bowei,Jiang, Lei,Wang, Qi,et al. Micropumps actuated negative pressure injection for microchip electrophoresis[J]. ELECTROPHORESIS,2008,29(24):4906-4913.
APA Li, Bowei,Jiang, Lei,Wang, Qi,Oin, Jianhua,Lin, Bingcheng,&林炳承.(2008).Micropumps actuated negative pressure injection for microchip electrophoresis.ELECTROPHORESIS,29(24),4906-4913.
MLA Li, Bowei,et al."Micropumps actuated negative pressure injection for microchip electrophoresis".ELECTROPHORESIS 29.24(2008):4906-4913.
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