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Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis
Wang, Fangjun; Dong, Jing; Ye, Mingliang; Wu, Ren'an; Zou, Hanfa; Zou HF(邹汉法)
关键词Methacrylate Porogenic Solvent Monolithic Column Shotgun Proteome Analysis Proteome Coverage Online Multidimensional Separation
刊名JOURNAL OF CHROMATOGRAPHY A
2009-05-01
ISSN0021-9673
DOI10.1016/j.chroma.2009.02.082
1216期:18页:3887-3894
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ELECTRON-CAPTURE DISSOCIATION ; RIGID POLYMER MONOLITHS ; CHROMATOGRAPHY-MASS SPECTROMETRY ; CAPILLARY LIQUID-CHROMATOGRAPHY ; STATIONARY PHASES ; NANOELECTROSPRAY IONIZATION ; IDENTIFICATION TECHNOLOGY ; SEPARATION MEDIA ; PEAK-CAPACITY ; TRAP COLUMN
英文摘要Monolithic columns are widely used in shotgun proteome analysis. However, it is difficult to increase the separation capability and proteome coverage by using conventionally organic polymer-based monolithic column due to the difficulty of controlling homogeneity of the overall pore structure (both pores and microglobules), which leads to relatively low column efficiency. Therefore, we studied the effect of constitute and percentage of porogenic solvent, functional monomer, column length, and separation gradient on the peak capacity and proteome coverage by methacrylate-based reversed phase monolithic columns. it was demonstrated that the porous property of the hydrophobic monolith, which was mainly determined by the porogenic solvent, was crucial to the proteome coverage when similar methacrylate monomer was utilized and a ternary porogenic solvent was adopted to prepare C12 monolithic column with relatively homogeneous overall pore structure. It was also shown that high proteome coverage could be reliably obtained with online multidimensional separation using totally monolithic columns system with the length of analytical column at 85 cm and reversed phase separation gradient at 210 min. (C) 2009 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000265699900028
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101615
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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Wang, Fangjun,Dong, Jing,Ye, Mingliang,et al. Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(18):3887-3894.
APA Wang, Fangjun,Dong, Jing,Ye, Mingliang,Wu, Ren'an,Zou, Hanfa,&邹汉法.(2009).Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis.JOURNAL OF CHROMATOGRAPHY A,1216(18),3887-3894.
MLA Wang, Fangjun,et al."Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis".JOURNAL OF CHROMATOGRAPHY A 1216.18(2009):3887-3894.
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