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题名: Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis
作者: Wang, Fangjun1;  Dong, Jing1;  Ye, Mingliang1;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: Methacrylate ;  Porogenic solvent ;  Monolithic column ;  Shotgun proteome analysis ;  Proteome coverage ;  Online multidimensional separation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-05-01
DOI: 10.1016/j.chroma.2009.02.082
卷: 1216, 期:18, 页:3887-3894
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Monolithic columns are widely used in shotgun proteome analysis. However, it is difficult to increase the separation capability and proteome coverage by using conventionally organic polymer-based monolithic column due to the difficulty of controlling homogeneity of the overall pore structure (both pores and microglobules), which leads to relatively low column efficiency. Therefore, we studied the effect of constitute and percentage of porogenic solvent, functional monomer, column length, and separation gradient on the peak capacity and proteome coverage by methacrylate-based reversed phase monolithic columns. it was demonstrated that the porous property of the hydrophobic monolith, which was mainly determined by the porogenic solvent, was crucial to the proteome coverage when similar methacrylate monomer was utilized and a ternary porogenic solvent was adopted to prepare C12 monolithic column with relatively homogeneous overall pore structure. It was also shown that high proteome coverage could be reliably obtained with online multidimensional separation using totally monolithic columns system with the length of analytical column at 85 cm and reversed phase separation gradient at 210 min. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: ELECTRON-CAPTURE DISSOCIATION ;  RIGID POLYMER MONOLITHS ;  CHROMATOGRAPHY-MASS SPECTROMETRY ;  CAPILLARY LIQUID-CHROMATOGRAPHY ;  STATIONARY PHASES ;  NANOELECTROSPRAY IONIZATION ;  IDENTIFICATION TECHNOLOGY ;  SEPARATION MEDIA ;  PEAK-CAPACITY ;  TRAP COLUMN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000265699900028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101615
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Fangjun,Dong, Jing,Ye, Mingliang,et al. Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(18):3887-3894.
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