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Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis
Wang, Fangjun; Dong, Jing; Ye, Mingliang; Wu, Ren'an; Zou, Hanfa; Zou HF(邹汉法)
KeywordMethacrylate Porogenic Solvent Monolithic Column Shotgun Proteome Analysis Proteome Coverage Online Multidimensional Separation
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2009-05-01
ISSN0021-9673
DOI10.1016/j.chroma.2009.02.082
Volume1216Issue:18Pages:3887-3894
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordELECTRON-CAPTURE DISSOCIATION ; RIGID POLYMER MONOLITHS ; CHROMATOGRAPHY-MASS SPECTROMETRY ; CAPILLARY LIQUID-CHROMATOGRAPHY ; STATIONARY PHASES ; NANOELECTROSPRAY IONIZATION ; IDENTIFICATION TECHNOLOGY ; SEPARATION MEDIA ; PEAK-CAPACITY ; TRAP COLUMN
AbstractMonolithic columns are widely used in shotgun proteome analysis. However, it is difficult to increase the separation capability and proteome coverage by using conventionally organic polymer-based monolithic column due to the difficulty of controlling homogeneity of the overall pore structure (both pores and microglobules), which leads to relatively low column efficiency. Therefore, we studied the effect of constitute and percentage of porogenic solvent, functional monomer, column length, and separation gradient on the peak capacity and proteome coverage by methacrylate-based reversed phase monolithic columns. it was demonstrated that the porous property of the hydrophobic monolith, which was mainly determined by the porogenic solvent, was crucial to the proteome coverage when similar methacrylate monomer was utilized and a ternary porogenic solvent was adopted to prepare C12 monolithic column with relatively homogeneous overall pore structure. It was also shown that high proteome coverage could be reliably obtained with online multidimensional separation using totally monolithic columns system with the length of analytical column at 85 cm and reversed phase separation gradient at 210 min. (C) 2009 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000265699900028
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/101615
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, Fangjun,Dong, Jing,Ye, Mingliang,et al. Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(18):3887-3894.
APA Wang, Fangjun,Dong, Jing,Ye, Mingliang,Wu, Ren'an,Zou, Hanfa,&邹汉法.(2009).Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis.JOURNAL OF CHROMATOGRAPHY A,1216(18),3887-3894.
MLA Wang, Fangjun,et al."Improvement of proteome coverage using hydrophobic monolithic columns in shotgun proteome analysis".JOURNAL OF CHROMATOGRAPHY A 1216.18(2009):3887-3894.
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