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题名: Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds
作者: Ren, Lianbing1;  Liu, Zhen1;  Dong, Mingming1;  Ye, Mingliang2;  Zou, Hanfa2
通讯作者: 邹汉法
关键词: Boronate affinity ;  Capillary HPLC ;  cis-Diol ;  Enrichment ;  Monolithic column
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-06-05
DOI: 10.1016/j.chroma.2009.04.036
卷: 1216, 期:23, 页:4768-4774
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2;4
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Boronate affinity chromatography (BAC) is an important tool for specific capture and separation of cis-diol-containing compounds such as glycoproteins, RNA and carbohydrates. Only a few reports on monolithic column-based BAC have appeared. In this paper, boronate functionalized monolithic capillary column was synthesized by in situ free radical polymerization for the first time. The prepared column was first characterized in terms of morphology, pore properties, capacity and retention mechanisms. The column exhibited uniform open channel network and high capture capacity. Systematical investigation on the retention mechanism revealed that multiple intermolecular interactions occur between the analytes and the boronate affinity monolith, including boronate affinity, reversed-phase, cation-exchange and hydrogen bonding interactions, depending on the conditions used. In addition. the presence of Lewis base Such as fluoride ion in the mobile phase was found to be favorable to the complexation between cis-diol-containing compounds with the boronic acid ligand under less basic conditions. On the basis of these fundamental investigations, the prepared monolithic column was then applied to the capture of adenosine and flavin adenine dinucleotide. The investigations in this Study provide sound understanding not only on how to manipulate the separation selectivity through selection of appropriate mobile phase composition on the currently prepared columns but also on how to design next-generation columns with desired properties and functions. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: SHOTGUN PROTEOME ANALYSIS ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  MALDI-TOF/TOF-MS ;  STATIONARY PHASES ;  REVERSED-PHASE ;  ESI-MS/MS ;  SEPARATION ;  COLUMNS ;  PROTEINS ;  ELECTROCHROMATOGRAPHY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000266449800009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101645
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nanjing Univ, Key Lab Analyt Chem Life Sci, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Ren, Lianbing,Liu, Zhen,Dong, Mingming,et al. Synthesis and characterization of a new boronate affinity monolithic capillary for specific capture of cis-diol-containing compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(23):4768-4774.
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