中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis
作者: Tao, Dingyin1, 2;  Zhu, Guajie1, 2;  Sun, Liangliang1, 2;  Ma, Junfeng1, 2;  Liang, Zhen1;  Zhang, Weibing1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: mu RPLC MS/MS ;  Serially coupled microcolumn ;  Shotgun proteome analysis
刊名: PROTEOMICS
发表日期: 2009-04-01
DOI: 10.1002/pmic.200800649
卷: 9, 期:7, 页:2029-2036
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Microcolumn RPLC (mu RPLC) is one of the optimum separation modes for shotgun proteomic analysis. To identify as many proteins as possible by MS/MS, the improvement on separation efficiency and peak capacity of mu RPLC is indispensable. Although the increase in column length is one of the effective solutions, the preparation of along microcolumn is rather difficult due to the high backpressure generated during the packing procedure. In our recent work, through connecting microcolumns of 5, 10, and 15 cm length via unions with minimal dead volume, long microcolumns with length up to 30 cm were obtained, with which 318 proteins were identified from proteins extracted from Escherichia coli by mu RPLC-ESI MS/MS, and similar distributions of M(w) and pI were found with single and various coupled microcolumns. Furthermore, by using MS/MS with improved sensitivity, with such a serially coupled 30 cm long microcolumn, 1692 proteins were identified within 7 h from rat brain tissue, with false positive rate (FPR) < 1%. All these results demonstrated that serially couple microcolumns might be of great promising to improve the separation capacity of mu RPLC in shotgun proteomic analysis.
关键词[WOS]: 2-DIMENSIONAL GEL-ELECTROPHORESIS ;  MONOLITHIC SILICA COLUMNS ;  TANDEM MASS-SPECTROMETRY ;  LOW-ABUNDANCE PROTEINS ;  ESCHERICHIA-COLI ;  PEAK-CAPACITY ;  PERFORMANCE ;  TEMPERATURE ;  TECHNOLOGY ;  AUTOMATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000265339000025
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101655
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Tao, Dingyin,Zhu, Guajie,Sun, Liangliang,et al. Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis[J]. PROTEOMICS,2009,9(7):2029-2036.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Tao, Dingyin]'s Articles
 [Zhu, Guajie]'s Articles
 [Sun, Liangliang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Tao, Dingyin]‘s Articles
 [Zhu, Guajie]‘s Articles
 [Sun, Liangliang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace