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Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis
Tao, Dingyin1,2; Zhu, Guajie1,2; Sun, Liangliang1,2; Ma, Junfeng1,2; Liang, Zhen1; Zhang, Weibing1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Mu Rplc Ms/ms Serially Coupled Microcolumn Shotgun Proteome Analysis
刊名PROTEOMICS
2009-04-01
DOI10.1002/pmic.200800649
9期:7页:2029-2036
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]2-DIMENSIONAL GEL-ELECTROPHORESIS ; MONOLITHIC SILICA COLUMNS ; TANDEM MASS-SPECTROMETRY ; LOW-ABUNDANCE PROTEINS ; ESCHERICHIA-COLI ; PEAK-CAPACITY ; PERFORMANCE ; TEMPERATURE ; TECHNOLOGY ; AUTOMATION
英文摘要Microcolumn RPLC (mu RPLC) is one of the optimum separation modes for shotgun proteomic analysis. To identify as many proteins as possible by MS/MS, the improvement on separation efficiency and peak capacity of mu RPLC is indispensable. Although the increase in column length is one of the effective solutions, the preparation of along microcolumn is rather difficult due to the high backpressure generated during the packing procedure. In our recent work, through connecting microcolumns of 5, 10, and 15 cm length via unions with minimal dead volume, long microcolumns with length up to 30 cm were obtained, with which 318 proteins were identified from proteins extracted from Escherichia coli by mu RPLC-ESI MS/MS, and similar distributions of M(w) and pI were found with single and various coupled microcolumns. Furthermore, by using MS/MS with improved sensitivity, with such a serially coupled 30 cm long microcolumn, 1692 proteins were identified within 7 h from rat brain tissue, with false positive rate (FPR) < 1%. All these results demonstrated that serially couple microcolumns might be of great promising to improve the separation capacity of mu RPLC in shotgun proteomic analysis.
语种英语
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WOS记录号WOS:000265339000025
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101655
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
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GB/T 7714
Tao, Dingyin,Zhu, Guajie,Sun, Liangliang,et al. Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis[J]. PROTEOMICS,2009,9(7):2029-2036.
APA Tao, Dingyin.,Zhu, Guajie.,Sun, Liangliang.,Ma, Junfeng.,Liang, Zhen.,...&张丽华.(2009).Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis.PROTEOMICS,9(7),2029-2036.
MLA Tao, Dingyin,et al."Serially coupled microcolumn reversed phase liquid chromatography for shotgun proteomic analysis".PROTEOMICS 9.7(2009):2029-2036.
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