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题名: Recent advances in immobilized enzymatic reactors and their applications in proteome analysis
作者: Ma, Junfeng1, 2;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Weibing1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Immobilized enzymes ;  Carrier ;  Proteome ;  Review
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2009-01-19
DOI: 10.1016/j.aca.2007.08.045
卷: 632, 期:1, 页:1-8
收录类别: SCI ;  ISTP
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Immobilized enzymatic reactors recently have drawn much attention because of the striking advantages, such as high substrate turnover rate and ease in coupling with the separation and detection systems. Carrier materials, which have great effects on the development of the immobilized enzymatic reactors, have always being the focus of study. In this paper, the contributions, mainly in the last 5 years, on the enzymatic reactors and their applications in proteome study are reviewed, with some newly developed inorganic and organic carriers for enzyme immobilization described in details. Moreover, the hyphenation of immobilized enzymatic reactors with the separation and identification systems is also summarized. By reviewing these achievements, it could be seen that enzymatic reactors have very bright future, especially in proteome analysis. (C) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TANDEM MASS-SPECTROMETRY ;  HIGHLY EFFICIENT PROTEOLYSIS ;  PEPTIDE-MAPPING ANALYSIS ;  LESS COMMON APPLICATIONS ;  SOLID-PHASE EXTRACTION ;  CAPILLARY-ELECTROPHORESIS ;  TRYPSIN MICROREACTOR ;  MONOLITHIC SILICA ;  AFFINITY-CHROMATOGRAPHY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000262585500001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101669
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Ma, Junfeng,Zhang, Lihua,Liang, Zhen,et al. Recent advances in immobilized enzymatic reactors and their applications in proteome analysis[J]. ANALYTICA CHIMICA ACTA,2009,632(1):1-8.
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