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题名: Preparation of Core-shell Magnetic Molecularly Imprinted Polymer Nanoparticles for Recognition of Bovine Hemoglobin
作者: Li, Lin1;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 2
通讯作者: 张玉奎
关键词: adsorption ;  imprinting ;  magnetic properties ;  nanoparticles ;  thin films
刊名: CHEMISTRY-AN ASIAN JOURNAL
发表日期: 2009
DOI: 10.1002/asia.200800300
卷: 4, 期:2, 页:286-293
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2;4
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: In this work, the core-shell bovine hemoglobin (BHb) imprinted magnetic nanoparticles (MNPs) with a mean diameter of 210 nm were synthesized for the first time. In this protocol, the initial step involved co-precipitation of Fe(2+) and Fe(3+) in an ammonia solution. Silica was then coated on the Fe(3)O(4), nanoparticles using a sol-gel method to obtain silica shell magnetic nanoparticles. Subsequently, 3-amino-phenylboronic acid (APBA), which is the functional and cross-linking monomer, and poly(APBA) thin films were coated onto the silica-modified Fe(3)O(4) surface through oxidation with ammonium persulfate in an aqueous solution in the presence or absence of protein. The morphology, adsorption, and recognition properties of the magnetic molecularly imprinted nanomaterial were investigated by transmission electron microscopy (TEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Rebinding experiments were carried out to establish the equilibrium time and to determine the specific binding capacity and selective recognition. The protein adsorption results showed that poly(APBA) MIPs-coated magnetic nanoparticles have high adsorption capacity for template protein BHb and comparatively low non-specific adsorption. The imprinted magnetic nanoparticles could easily reach the adsorption equilibrium and magnetic separation under an external magnetic field, thus avoiding problems related to the bulk polymer. We believe that the imprinted polymercoated magnetic nanoparticles can be one of the most promising candidates for various applications, which include chemical and biochemical separation, cell sorting, recognition elements in biosensors, and drug delivery.
关键词[WOS]: IRON-OXIDE NANOPARTICLES ;  PROTEIN RECOGNITION ;  SERUM-ALBUMIN ;  THIN-FILMS ;  SURFACE ;  MICROSPHERES ;  ADSORPTION ;  BEADS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000263372700009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101673
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Li, Lin,He, Xiwen,Chen, Langxing,et al. Preparation of Core-shell Magnetic Molecularly Imprinted Polymer Nanoparticles for Recognition of Bovine Hemoglobin[J]. CHEMISTRY-AN ASIAN JOURNAL,2009,4(2):286-293.
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