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Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins
Qin, Lei1; He, Xi-Wen1; Zhang, Wei1; Li, Wen-You1; Zhang, Yu-Kui1,2; Zhang YK(张玉奎)
KeywordMolecular Imprinting Protein Separation Polystyrene Beads Iniferter Chromatography
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2009-01-30
DOI10.1016/j.chroma.2008.12.007
Volume1216Issue:5Pages:807-814
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank2;5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordELECTROSTATIC FUNCTIONAL-GROUPS ; RECOGNITION ; COPOLYMERIZATION ; ANTIBODIES ; FILMS ; GELS
AbstractA new and facile fabricating method for lysozyme molecularly imprinted polymer beads (lysozyme-MIP beads) in aqueous media was presented, Mesoporous chloromethylated polystyrene beads (MCP beads) containing dithiocarbamate iniferter (initiator transfer agent terminator) were used as supports for the grafting of lysozyme imprinted copolymers with acrylamide and N,N'-methylenebisacrylamide through surface initiated living-radical polymerization (SIP). After the polymerization, a layer of lysozyme-MIP was formed on the MCP beads. The SIP allowed an efficient control of the grafting process and suppressed solution propagation. Therefore, the obtained lysozyme-MIP beads had a large quantity of well-distributed pores on the surface without any visible gel formation in solution and were more advantageous comparing with traditional MIPs which were prepared by traditionally initiated radical polymerization. The obtained composites were characterized by Fourier transform infrared spectroscopy, elemental analysis, nitrogen sorption analysis and scanning electron microscopy. Chromatographic behaviors of the column packed with lysozyme-MIP beads exhibited ability in separating lysozyme from competitive protein (bovine hemoglobin, bovine serum albumin, ovalbumin or cytochrome c) in aqueous mobile phase. (C) 2008 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000262797100008
Citation statistics
Cited Times:98[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/101675
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang YK(张玉奎)
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Qin, Lei,He, Xi-Wen,Zhang, Wei,et al. Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(5):807-814.
APA Qin, Lei,He, Xi-Wen,Zhang, Wei,Li, Wen-You,Zhang, Yu-Kui,&张玉奎.(2009).Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins.JOURNAL OF CHROMATOGRAPHY A,1216(5),807-814.
MLA Qin, Lei,et al."Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins".JOURNAL OF CHROMATOGRAPHY A 1216.5(2009):807-814.
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