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Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins
Qin, Lei1; He, Xi-Wen1; Zhang, Wei1; Li, Wen-You1; Zhang, Yu-Kui1,2; Zhang YK(张玉奎)
关键词Molecular Imprinting Protein Separation Polystyrene Beads Iniferter Chromatography
刊名JOURNAL OF CHROMATOGRAPHY A
2009-01-30
DOI10.1016/j.chroma.2008.12.007
1216期:5页:807-814
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名2;5
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ELECTROSTATIC FUNCTIONAL-GROUPS ; RECOGNITION ; COPOLYMERIZATION ; ANTIBODIES ; FILMS ; GELS
英文摘要A new and facile fabricating method for lysozyme molecularly imprinted polymer beads (lysozyme-MIP beads) in aqueous media was presented, Mesoporous chloromethylated polystyrene beads (MCP beads) containing dithiocarbamate iniferter (initiator transfer agent terminator) were used as supports for the grafting of lysozyme imprinted copolymers with acrylamide and N,N'-methylenebisacrylamide through surface initiated living-radical polymerization (SIP). After the polymerization, a layer of lysozyme-MIP was formed on the MCP beads. The SIP allowed an efficient control of the grafting process and suppressed solution propagation. Therefore, the obtained lysozyme-MIP beads had a large quantity of well-distributed pores on the surface without any visible gel formation in solution and were more advantageous comparing with traditional MIPs which were prepared by traditionally initiated radical polymerization. The obtained composites were characterized by Fourier transform infrared spectroscopy, elemental analysis, nitrogen sorption analysis and scanning electron microscopy. Chromatographic behaviors of the column packed with lysozyme-MIP beads exhibited ability in separating lysozyme from competitive protein (bovine hemoglobin, bovine serum albumin, ovalbumin or cytochrome c) in aqueous mobile phase. (C) 2008 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000262797100008
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101675
专题中国科学院大连化学物理研究所
通讯作者Zhang YK(张玉奎)
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Qin, Lei,He, Xi-Wen,Zhang, Wei,et al. Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(5):807-814.
APA Qin, Lei,He, Xi-Wen,Zhang, Wei,Li, Wen-You,Zhang, Yu-Kui,&张玉奎.(2009).Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins.JOURNAL OF CHROMATOGRAPHY A,1216(5),807-814.
MLA Qin, Lei,et al."Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins".JOURNAL OF CHROMATOGRAPHY A 1216.5(2009):807-814.
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