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题名: Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins
作者: Qin, Lei1;  He, Xi-Wen1;  Zhang, Wei1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
通讯作者: 张玉奎
关键词: Molecular imprinting ;  Protein separation ;  Polystyrene beads ;  Iniferter ;  Chromatography
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-01-30
DOI: 10.1016/j.chroma.2008.12.007
卷: 1216, 期:5, 页:807-814
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2;5
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new and facile fabricating method for lysozyme molecularly imprinted polymer beads (lysozyme-MIP beads) in aqueous media was presented, Mesoporous chloromethylated polystyrene beads (MCP beads) containing dithiocarbamate iniferter (initiator transfer agent terminator) were used as supports for the grafting of lysozyme imprinted copolymers with acrylamide and N,N'-methylenebisacrylamide through surface initiated living-radical polymerization (SIP). After the polymerization, a layer of lysozyme-MIP was formed on the MCP beads. The SIP allowed an efficient control of the grafting process and suppressed solution propagation. Therefore, the obtained lysozyme-MIP beads had a large quantity of well-distributed pores on the surface without any visible gel formation in solution and were more advantageous comparing with traditional MIPs which were prepared by traditionally initiated radical polymerization. The obtained composites were characterized by Fourier transform infrared spectroscopy, elemental analysis, nitrogen sorption analysis and scanning electron microscopy. Chromatographic behaviors of the column packed with lysozyme-MIP beads exhibited ability in separating lysozyme from competitive protein (bovine hemoglobin, bovine serum albumin, ovalbumin or cytochrome c) in aqueous mobile phase. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: ELECTROSTATIC FUNCTIONAL-GROUPS ;  RECOGNITION ;  COPOLYMERIZATION ;  ANTIBODIES ;  FILMS ;  GELS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000262797100008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101675
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Qin, Lei,He, Xi-Wen,Zhang, Wei,et al. Surface-modified polystyrene beads as photografting imprinted polymer matrix for chromatographic separation of proteins[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(5):807-814.
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