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题名: Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength
作者: Cong, Yongzheng1, 2;  Liang, Yu1;  Zhang, Lihua1;  Zhang, Weibing1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: IEF ;  Microchip ;  Protein separation ;  Stepwise gradient of electric field strength
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-02-01
DOI: 10.1002/jssc.200800514
卷: 32, 期:3, 页:462-465
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A stepwise gradient of electric field strength was proposed for microchip IEF-based protein separation, by which after focusing at low voltages, IEF was performed by applying higher separation voltages step-by-step. A linear relationship between the focusing time and the inverse of the electric field strength was found. In addition, the conductivity of an established pH gradient showed a negative but nonlinear correlation with the applied voltage. Based on the above-mentioned results, a stepwise gradient of electric field strength, ranging from 160 to 1500 V/cm was applied in the separation of proteins extracted from Escherichia coli in a straight glass microchip channel permanently coated by polyacrylamide. Compared to the conventional separation performed under a constant field strength of 750 V/cm, the increased stepwise gradient of electric field strength resulted in improved resolution and decreased focusing time, while without the negative effects of joule heat for protein separation. All these results demonstrated that such a method might be of great significance to achieve high resolution and high-throughput analysis of complex protein samples for microchip IEF.
关键词[WOS]: PLASTIC MICROFLUIDIC DEVICES ;  ELECTROPHORESIS ;  CHIP ;  MICROCHANNELS ;  ADAPTATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000263385600018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101679
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Cong, Yongzheng,Liang, Yu,Zhang, Lihua,et al. Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(3):462-465.
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