DICP OpenIR
Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength
Cong, Yongzheng1,2; Liang, Yu1; Zhang, Lihua1; Zhang, Weibing1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Ief Microchip Protein Separation Stepwise Gradient Of Electric Field Strength
刊名JOURNAL OF SEPARATION SCIENCE
2009-02-01
DOI10.1002/jssc.200800514
32期:3页:462-465
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PLASTIC MICROFLUIDIC DEVICES ; ELECTROPHORESIS ; CHIP ; MICROCHANNELS ; ADAPTATION
英文摘要A stepwise gradient of electric field strength was proposed for microchip IEF-based protein separation, by which after focusing at low voltages, IEF was performed by applying higher separation voltages step-by-step. A linear relationship between the focusing time and the inverse of the electric field strength was found. In addition, the conductivity of an established pH gradient showed a negative but nonlinear correlation with the applied voltage. Based on the above-mentioned results, a stepwise gradient of electric field strength, ranging from 160 to 1500 V/cm was applied in the separation of proteins extracted from Escherichia coli in a straight glass microchip channel permanently coated by polyacrylamide. Compared to the conventional separation performed under a constant field strength of 750 V/cm, the increased stepwise gradient of electric field strength resulted in improved resolution and decreased focusing time, while without the negative effects of joule heat for protein separation. All these results demonstrated that such a method might be of great significance to achieve high resolution and high-throughput analysis of complex protein samples for microchip IEF.
语种英语
原文出处查看原文
WOS记录号WOS:000263385600018
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/101679
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Cong, Yongzheng,Liang, Yu,Zhang, Lihua,et al. Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(3):462-465.
APA Cong, Yongzheng,Liang, Yu,Zhang, Lihua,Zhang, Weibing,Zhang, Yukui,&张丽华.(2009).Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength.JOURNAL OF SEPARATION SCIENCE,32(3),462-465.
MLA Cong, Yongzheng,et al."Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength".JOURNAL OF SEPARATION SCIENCE 32.3(2009):462-465.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cong, Yongzheng]的文章
[Liang, Yu]的文章
[Zhang, Lihua]的文章
百度学术
百度学术中相似的文章
[Cong, Yongzheng]的文章
[Liang, Yu]的文章
[Zhang, Lihua]的文章
必应学术
必应学术中相似的文章
[Cong, Yongzheng]的文章
[Liang, Yu]的文章
[Zhang, Lihua]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。