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题名: MEKC-LIF analysis of rhodamine123 delivered by carbon nanotubes in K562 cells
作者: Li, Ruibin1;  Wu, Ren'an1;  Wu, Minghuo1;  Zou, Hanfa1;  Ma, Hong2;  Yang, Ling2;  Le, X. Chris3
通讯作者: 邹汉法
关键词: Capillary electrophoresis ;  Carbon nanotubes ;  Fluorescence quenching ;  Drug delivery ;  K562 cell
刊名: ELECTROPHORESIS
发表日期: 2009-06-01
DOI: 10.1002/elps.200800737
卷: 30, 期:11, 页:1906-1912
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Oxidized single-walled carbon nanotubes (o-SWNTs) were employed as the drug carriers to deliver the small molecules of Rhodamine123 (Rho123) into the K562 cells via physical adsorption. However, due to the fluorescence quenching of Rho123 on carbon nanotubes, the quantitative determination of Rho123 in cells is difficult. Based on the MEKC coupled with LIF detection, a quantitative approach was developed for the determination of Rho123 delivered into K562 cells by o-SWNTs. Where the adsorbed Rho123 on o-SWNTs could be desorbed by SDS in running buffer and be simultaneously separated with o-SWNTs due to the differences of their electrophoretic mobility by applying the electric potential at the both ends of capillary. Using this approach, the intracellular uptakes of Rho123 in multidrug-resistant and multidrug-sensitive leukemia cells were quantified, and the results showed that o-SWNTs could be used as the potential drug carriers to deliver small molecules into cells via the physical adsorption along with the circumventing of multidrug resistance of leukemia cells.
关键词[WOS]: MULTIDRUG-RESISTANCE ;  FLOW-CYTOMETRY ;  TRANSPORTERS ;  DIAGNOSIS ;  DNA ;  CE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000267689900008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101707
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
3.Univ Alberta, Fac Med, Dept Publ Hlth Sci, Edmonton, AB, Canada

Recommended Citation:
Li, Ruibin,Wu, Ren'an,Wu, Minghuo,et al. MEKC-LIF analysis of rhodamine123 delivered by carbon nanotubes in K562 cells[J]. ELECTROPHORESIS,2009,30(11):1906-1912.
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