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题名: Integration of monolithic frit into the particulate capillary (IMFPC) column in shotgun proteome analysis
作者: Wang, Fangjun1;  Dong, Jing1;  Ye, Mingliang1;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: Monolithic frit ;  Nano-flow liquid chromatography ;  Particulate column ;  Fritless column ;  Mouse liver
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2009-10-12
DOI: 10.1016/j.aca.2009.06.066
卷: 652, 期:1-2, 页:324-330
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Capillary column plays an important role in nano-flow liquid chromatography coupled with tandem mass spectrometry for dealing with the high dynamic range and complexity of protein samples in shotgun proteome analysis. In this study, the integrated monolithic frit into the particulate capillary (IMFPC) column was prepared. By comparing the prepared IMFPC column with conventionally fritless capillary column, smaller size of packing materials could be easily packed into the capillary to achieve higher average peak capacity and proteome coverage. As the monolithic emitter was integrated onto this type of column, the void volume between packing particles and electrospray emitter was eliminated and the electrospray quality was improved. The prepared IMFPC column was applied to proteome analysis of mouse liver extracts, and it was observed that the number of identified proteins and peptides increased 14.9% and 12.9% as well as the peak capacity increased 11.6% by using IMFPC column over conventionally fritless capillary column. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: ELECTROSPRAY MASS-SPECTROMETRY ;  LIQUID-CHROMATOGRAPHY ;  NANOELECTROSPRAY IONIZATION ;  MULTIDIMENSIONAL CHROMATOGRAPHY ;  IDENTIFICATION TECHNOLOGY ;  STATIONARY PHASES ;  MS/MS ;  ELECTROCHROMATOGRAPHY ;  SEPARATION ;  PEPTIDES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000270759800034
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101817
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Fangjun,Dong, Jing,Ye, Mingliang,et al. Integration of monolithic frit into the particulate capillary (IMFPC) column in shotgun proteome analysis[J]. ANALYTICA CHIMICA ACTA,2009,652(1-2):324-330.
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