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题名: Mesoporous silica hollow sphere (MSHS) for the bioelectrochemistry of horseradish peroxidase
作者: Cao, Zhaoxia1, 2;  Zhang, Jian1, 2;  Zeng, Julan1, 3;  Sun, Lixian1;  Xu, Fen1;  Cao, Zhong3;  Zhang, Ling3;  Yang, Daowu3
通讯作者: 孙立贤
关键词: Hollow mesoporous silica spheres ;  Horseradish peroxidase ;  Biosensor ;  Direct electron transfer
刊名: TALANTA
发表日期: 2009-01-15
DOI: 10.1016/j.talanta.2008.06.043
卷: 77, 期:3, 页:943-947
收录类别: SCI
文章类型: Article
部门归属: 15
项目归属: 1504
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: In this work, novel mesoporous silica hollow spheres (MSHS) were chosen as an immobilization matrix, to construct a mediator-free third-generation HRP biosensor. UV-vis spectroscopy revealed that horseradish peroxidase (HRP) entrapped in MSHS could retain its native Structure. FTIR spectroscopy and nitrogen adsorption-desorption isotherms indicated that HRP are intercalated into the mesopores. The direct electron transfer of HRP entrapped in MSHS was observed. A pair of stable and well-defined redox peaks of HRP with a formal potential of about -0.150V (vs. Ag/AgCl) in 0.1 M pH 7.0 phosphate-buffered solution (PBS) were obtained. The biosensor exhibited a fast amperometric response to H(2)O(2) with a linear range of 3.9 x 10(-6) to 1.4 x 10(-4) M (R = 0.997, N = 20). The detection limit was 1.2 x 10(-6) M based S/N = 3. (C) 2008 Published by Elsevier B.V.
关键词[WOS]: DIRECT ELECTROCHEMISTRY ;  HYDROGEN-PEROXIDE ;  ENZYME IMMOBILIZATION ;  LYSOZYME ADSORPTION ;  CATALYTIC ACTIVITY ;  H2O2 BIOSENSOR ;  GOLD ELECTRODE ;  MATRIX ;  NANOPARTICLES ;  ENCAPSULATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000262761300002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101849
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat &Thermochem Lab, Dalian 116023, Liaoning Prov, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Changsha Univ Sci & Technol, Sch Chem & Environm Engn, Changsha 410076, Hunan, Peoples R China

Recommended Citation:
Cao, Zhaoxia,Zhang, Jian,Zeng, Julan,et al. Mesoporous silica hollow sphere (MSHS) for the bioelectrochemistry of horseradish peroxidase[J]. TALANTA,2009,77(3):943-947.
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