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题名: Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO-gold nanoparticle-Nafion nanocomposite
作者: Xiang, Cuili2;  Zou, Yongjin2;  Sun, Li-Xian1;  Xu, Fen3
通讯作者: 孙立贤 ;  徐芬
关键词: Horseradish peroixdase ;  Flowerlike ZnO ;  Gold nanoparticles ;  Direct electron transfer ;  Biosensor
刊名: SENSORS AND ACTUATORS B-CHEMICAL
发表日期: 2009-02-02
DOI: 10.1016/j.snb.2008.10.058
卷: 136, 期:1, 页:158-162
收录类别: SCI
文章类型: Article
部门归属: 15
项目归属: 1504
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Electrochemistry ;  Instruments & Instrumentation
研究领域[WOS]: Chemistry ;  Electrochemistry ;  Instruments & Instrumentation
英文摘要: We developed a new flowerlike ZnO-gold nanoparticles (GNPs)-Nafion nanocomposite which can promote the direct electron transfer of horseradish peroxidase (HRP) immobilized in the film effectively. The cyclic voltammetric results show that HRP entrapped in the composite could undergo a direct quasi-reversible electrochemical reaction with a formal potential (E-0') of -0.32V (versus Ag/AgCl) in phosphate buffer solution (PBS). The flowerlike ZnO and GNPs show synergistic effect and the ZnO-GNPs-Nafion-HRP modified GC electrode gives an enhanced electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2). The calculated apparent Michaelis-Menten constant (K-m(app)) is M 1.76 mM. which is much lower than that reported, indicating a high catalytic activity of HRP. The catalysis currents increase linearly to the H2O2 concentration in a wide range of 1.5 x 10(-5) to 1.1 X 10(-3) M, with a correlation coefficient of 0.998. The detection limit is 9.0 x 10(-6) M (at the ratio of signal to noise, S/N = 3), demonstrating that the formed film provides a favorable microenvironment for the enzyme to retain its activity. Moreover, the modified electrode displays rapid response to H2O2 and possesses good stability and reproducibility. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: DIRECT ELECTRON-TRANSFER ;  GLASSY-CARBON ELECTRODE ;  SOL-GEL ;  BIOSENSOR ;  HEMOGLOBIN ;  NANOTUBES ;  FILM ;  IMMOBILIZATION ;  COMPOSITES ;  TYROSINASE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000263494200025
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101855
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Liaoning Normal Univ, Coll Chem & Chem Engn, Dalian 116029, Peoples R China

Recommended Citation:
Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,et al. Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO-gold nanoparticle-Nafion nanocomposite[J]. SENSORS AND ACTUATORS B-CHEMICAL,2009,136(1):158-162.
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