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题名: Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro
作者: Xiong, Chuannan1, 3;  Wu, Haige1, 2;  Wei, Peng1, 3;  Pan, Ma1, 3;  Tuo, Yaqin1;  Kusakabe, Isao4;  Du, Yuguang1
通讯作者: 杜昱光
关键词: Deacetylated chitohexaose ;  Angiogenesis ;  ECV304 ;  Vascular endothelial growth factor ;  Urokinase plasminogen activator
刊名: CARBOHYDRATE RESEARCH
发表日期: 2009-10-12
DOI: 10.1016/j.carres.2009.06.036
卷: 344, 期:15, 页:1975-1983
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1805
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ;  Chemistry, Applied ;  Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: This study was performed to demonstrate the effects of deacetylated chitohexaose (hexamer) separated from a chitooligosaccharide (COS) mixture on tumor angiogenesis and its mechanism of action. Five fractions from dimer to hexamer were separated by a linear gradient solution of HCl on a cation-exchange resin. Then HCl was removed from the fractions by a charcoal column. The purity of the five fractions was analyzed by HPLC and the molecular masses were analyzed by MALDI-TOFMS. The hexamer expressed an inhibitory influence on CAM angiogenesis in a dose-dependent manner at concentrations of 6.25-50 mu g/egg. On further investigation, we found that the hexamer had no toxic effect on normal ECV304 cells, but could inhibit the proliferation and migration of tumor-induced ECV304 cells in a dose-dependent manner. The mechanism was demonstrated through the detection of mRNA expression of VEGF, MMP-9, TIMP-1, TIMP-2 and uPA by RT-PCR, which showed that the hexamer down-regulated the VEGF and uPA mRNA expressions in ECV304 cells, but up-regulated the TIMP-1 mRNA expression. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ;  CHICK CHORIOALLANTOIC MEMBRANE ;  MATRIX METALLOPROTEINASES ;  PHYTOPHTHORA-CAPSICI ;  TUMOR ANGIOGENESIS ;  ANTI-ANGIOGENESIS ;  OLIGOCHITOSAN ;  CELLS ;  INDUCTION ;  STRATEGIES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000271043300007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/101905
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ, Bioengn Coll, Dalian 116622, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
4.Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 305, Japan

Recommended Citation:
Xiong, Chuannan,Wu, Haige,Wei, Peng,et al. Potent angiogenic inhibition effects of deacetylated chitohexaose separated from chitooligosaccharides and its mechanism of action in vitro[J]. CARBOHYDRATE RESEARCH,2009,344(15):1975-1983.
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